Articles producció científica> Bioquímica i Biotecnologia

Yeasts population dynamics during the spontaneous fermentation of white grape variety Malvasia

  • Datos identificativos

    Identificador: imarina:9383930
    Autores:
    Berradre, MSulbaran, BOjeda de Rodriguez, GFernandez, VMartinez, JEsteve-Zarzoso, B
    Resumen:
    Identification and characterization of natural yeast were done to the yeast isolated from a spontaneous fermentation of grape must (variety Malavasia) coming from the state of Zulia. Monitoring fermentation was done by determination of density, ethanol percentage; yeast cells were counted on optical microscopy and YEPD or Sabouraud agar plates. Identification of yeasts isolated was conducted by the amplification by PCR of the ribosomal gene 5.8S and intergenic transcribed spacers adjacent (ITS1 and ITS2), using the primers ITS1 and ITS4. The amplified products were analyzed by restriction enzymes Hinf I, Hae III, CfoI and DdeI. Yeast characterization Saccharomyces was done by restriction analysis of the mitochondrial DNA using the enzyme HinfI yeasts isolated during spontaneous fermentation belong to the species Hanseniaspora guillermondi, H. uvarum, Rhodotorula mucilaginosa and S. cerevisiae. During the first days of fermentation the main yeasts isolated belong to the genus Hanseniaspora, but from the eighth day of fermentation is fully imposed the specie S. cerevisie, one strain of this specie that was isolated until the end of fermentation.
  • Otros:

    Autor según el artículo: Berradre, M; Sulbaran, B; Ojeda de Rodriguez, G; Fernandez, V; Martinez, J; Esteve-Zarzoso, B
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: Esteve Zarzoso, Braulio
    Palabras clave: Autochtonous yeast Identification Prc-rflp 5.8 Prc-rflp 5.8s Regio Rflp analysis Spontaneous fermentation Wine yeast
    Resumen: Identification and characterization of natural yeast were done to the yeast isolated from a spontaneous fermentation of grape must (variety Malavasia) coming from the state of Zulia. Monitoring fermentation was done by determination of density, ethanol percentage; yeast cells were counted on optical microscopy and YEPD or Sabouraud agar plates. Identification of yeasts isolated was conducted by the amplification by PCR of the ribosomal gene 5.8S and intergenic transcribed spacers adjacent (ITS1 and ITS2), using the primers ITS1 and ITS4. The amplified products were analyzed by restriction enzymes Hinf I, Hae III, CfoI and DdeI. Yeast characterization Saccharomyces was done by restriction analysis of the mitochondrial DNA using the enzyme HinfI yeasts isolated during spontaneous fermentation belong to the species Hanseniaspora guillermondi, H. uvarum, Rhodotorula mucilaginosa and S. cerevisiae. During the first days of fermentation the main yeasts isolated belong to the genus Hanseniaspora, but from the eighth day of fermentation is fully imposed the specie S. cerevisie, one strain of this specie that was isolated until the end of fermentation.
    Áreas temáticas: Agronomy Agronomy and crop science Animal science and zoology Ciências agrárias i Ciências ambientais Ciências biológicas i Ciencias sociales Food science Plant science
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: braulio.esteve@urv.cat braulio.esteve@urv.cat
    ISSN: 0378-7818
    Identificador del autor: 0000-0001-6467-7086 0000-0001-6467-7086
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://produccioncientificaluz.org/index.php/agronomia/article/view/27066
    Referencia al articulo segun fuente origial: Revista De La Facultad De Agronomia De La Universidad Del Zulia. 29 (3): 453-474
    Referencia de l'ítem segons les normes APA: Berradre, M; Sulbaran, B; Ojeda de Rodriguez, G; Fernandez, V; Martinez, J; Esteve-Zarzoso, B (2012). Yeasts population dynamics during the spontaneous fermentation of white grape variety Malvasia. Revista De La Facultad De Agronomia De La Universidad Del Zulia, 29(3), 453-474
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2012
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Agronomy,Agronomy and Crop Science,Animal Science and Zoology,Food Science,Plant Science
    Autochtonous yeast
    Identification
    Prc-rflp 5.8
    Prc-rflp 5.8s
    Regio
    Rflp analysis
    Spontaneous fermentation
    Wine yeast
    Agronomy
    Agronomy and crop science
    Animal science and zoology
    Ciências agrárias i
    Ciências ambientais
    Ciências biológicas i
    Ciencias sociales
    Food science
    Plant science
    0378-7818
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