Development of techniques for the analysis of acetic acid bacteria populations and their interaction in different food environments

Identificador:  TDX:1440

Handle: https://hdl.handle.net/20.500.11797/TDX1440

Autors:  Valera Martínez, María José

Resum:
The development of molecular techniques for detection, identification and typing of Acetic Acid Bacteria is the key for a better understanding of the coexistence of this microbiota in food products. Different culture-dependent and -independent techniques were applied for the identification and the enumeration of Acetic Acid Bacteria microbiota present on healthy grapes and in wine of the Canary Islands as well as in strawberry vinegar biofilm. Species and genera of AAB not previously reported in these niches were detected. The design of specific primers and TaqMan-MGB probes in the 16S-23S rRNA gene internal transcribed region was successfully performed for the detection of the closely related species of Acetobacter malorum and Acetobacter cerevisiae using Real-Time PCR. The use of degenerated primers allowed the detection of cellulose synthase gene and the correlation of this gene with the cellulose production phenotype in AAB strains of different species. On the other hand, the functional screening in the genome of Komagataeibacter europaeus Gae02 allowed the detection of one protein, homologous to prephenate dehydratase, with quorum quenching activity.