Controlled growth of hydroxyapatite in the specific binding peptide

Identifier:  TFG:1217

Handle: https://hdl.handle.net/20.500.11797/TFG1217

Authors:  Mercè Minguet, Marta

Abstract:
Hydroxyapatite (HAp) materials have been an important area of development in the last decades due to their biomedical application as it is the main inorganic compound of bone. This current study tries to investigate the different effects of peptides binding to HAp. Four peptides known to promote HAp formation (CaP(V)-VTKHLNQISQSY, CaP(A)-APWHLSSQYSRT, CaP(S)-STLPIPHEFSRE, CaP(H)-SVSVGMKPSPRP) were successfully synthesised. Two wet chemical methods were explored for HAp growth. In method 1, calcium phosphate with the target phase (HAp) was obtained. However, in method 2, HAp was not obtained, instead the Brushite phase formed. CaP(V), CaP(A) and CaP(S), are the peptides that favoured the formation of HAp without the presence of other phases. CaP(H) results were not reproducible. Due to there being no significant differences in X-Ray Diffraction data, no conclusions were reached about the effects of each peptide.