Study of gene expression in edema induced in diabetic mice

Identifier:  TFG:1634

Handle: https://hdl.handle.net/20.500.11797/TFG1634

Authors:  Bañuls Tornero, Marc

Abstract:
Nowadays there are 425 million people with diabetes, which have a 25% chance of developing a diabetic foot ulcer, a complication that can lead to amputation of the limb. Therefore, several studies focus on investigating the improvement of the cure rate in these chronic wounds. One of the main methods to improve wound healing is to use scaffolds that contain compounds that improve the expression of genes actively involved in the healing of wounds (which in diabetic people are observed repressed). In our study we wanted to observe the effect of a bioactive compound added to a polylactic acid scaffold in the expression of the COL1A1 and VEGF-A genes. To do this, samples of ulcer tissue from mice were obtained and total RNA was obtained from these to perform an RT-qPCR. The results obtained suggest that the bioactive compound used leads to a repression of both genes. But it should be noted an important limitation in its interpretation. This is due to the low statistical validity due to the small number of samples, and the limited personal experience in the procedures performed.