Developing new silk based composite materials for dental and bone repair

Identifier:  TFG:1699

Handle: https://hdl.handle.net/20.500.11797/TFG1699

Authors:  Giménez Gómez, Alberto

Abstract:
In this project the main issue is the study of one of the chains that form the natural silk fibroin, which is named light chain. Bibliographical research about which is the best way to functionalise it has been done, in order to improve its properties as a biomaterial for medical purposes. Although any procedure to do this functionalization has been achieved the project has continued to the second part of the objective. In this part the light chain has been studied thoroughly in terms of secondary structure and stabilization factors. A short enough (37mer) sequence has been found, so it has been feasible to synthesize it with state of the art techniques (SPPS). This peptide has been purified (HPLC) and its presence has been confirmed (MALDI-TOF). Further investigations will be done in this field, like complete characterization (CD-UV, NMR, IR), and stability in in vivo medium.