Identifier: TFG:2951
Authors: del Palacio Rodríguez, Jose Luis
Abstract:
Fungi are organisms capable of degrading a wide variety of materials. The production of enzymes and secondary metabolites necessary to develop their metabolism is what has promoted the interest of the biotech industry in these organisms. Soil, as the main reservoir of fungal propagules, is where the prospecting for fungi that produce bioactive substances has been focused. However, mass sequencing techniques have shown that river sediments are a habitat of high fungal diversity, which has been neglected to date. So it is necessary to improve the techniques for the isolation in culture of these organisms and thus study their metabolism and extrolites. Our main objective is to contribute to the knowledge of the mycobiota of the river sediments in Spain. For this, various isolation techniques, direct seeding and activation of the latent spores present in fluvial sediments of two rivers were applied: Fondón from Chapela, Vigo and Segre, Lleida. More than 150 strains of filamentous fungi were isolated in pure culture. Of these, 57 produced some type of sporulation, so a total of 18 genera could be morphologically identified, the most frequent being Penicillium and Talaromyces, followed by others previously reported for this habitat such as Alternaria, Aspergillus, Chaetomium, Cladospoorium, Ulocladium, among others. . In this work, a new species of Penicillium, Section Roquefortorum, Penicillium gallaecium sp. nov., which has been approached from polyphasic taxonomy (morphologyphysiology- molecular phylogeny).