Modulation of TLR4-induced variations in mitochondrial CPT1 and CPT2 expression by immunomodulatory metabolites and its correlation on the selective functional differentiation of macrophages

Identifier:  TFG:4347

Handle: https://hdl.handle.net/20.500.11797/TFG4347

Authors:  Herrera Azcona, Aitana

Abstract:
Immunometabolism is a critical determinant in the activation of immune responses. Carnitine-palmitoyltransferases (CPTs) can regulate the immunometabolic effects by innate immune receptors. The objective of this study was to evaluate the potential of butyrate, itaconate and plant protease inhibitors (PPIs) to modulate TLR4-induced by LPS response on human macrophages. Butyrate and itaconate differentially affected mitochondrial metabolism. The sequential exposure to butyrate or PPIs and LPS, improved the mitochondrial activities of macrophages. All the treatments promoted higher variations in CPT2 expression than in CPT1. Butyrate and itaconate with LPS enhanced proinflammatory macrophages (CD68+) and favoured downward trends in CPT2 expression.