Diagnostic discordance in Neisseria gonorrhoeae: negative culture and positive PCR

Identifier:  TFG:5717

Handle: https://hdl.handle.net/20.500.11797/TFG5717

Authors:  Martínez Guindo, Sandra

Abstract:
Gonorrhea is the second most prevalent sexually transmitted disease. Microscopic inspection of Gram-stained tissues, bacterial culture, and PCR are required for the diagnosis. The aim of the study is to analyze the cases of positive PCR and negative culture for N. gonorrhoeae detected during the years 2018-2022 in the Clínico-Malvarrosa (Valencia). To identify possible causes, a retrospective analysis was performed using binary logistic regression using IBM SPSS software.The absence of symptoms in the patient at the time of sampling, a CT>27 (CT: cycle of quantification) on PCR, and antibiotic administration are the most important aspects that have been described.