An Enzyme-based Potentiometric Redox Biosensor for Glucose

Identifier:  TFM:126

Handle: https://hdl.handle.net/20.500.11797/TFM126

Authors:  Hoekstra, Rafael

Abstract:
A potentiometric sensor for the determination of glucose at clinically relevant levels is presented. This approach uses an electropolymerized membrane containing glucose oxidase immobilized on gold nanoparticles deposited on platinum or platinized paper. The paper-based biosensor showed a linear dependence with the logarithm of the glucose concentration in the range 10-4.5 M to 10-3 M and a sensitivity of -44.5 ± 3.7 mV/log[glucose] in dilute artificial serum at pH 7.4. Under these conditions, a response time of 22 s and a limit of detection (LOD) of 10-4.9 M were obtained. Experimental evidence suggests that the working principle of this sensor is based on monitoring changes in the local redox potential produced as a result of the enzymatic reaction. This approach offers a simpler, and more cost-effective alternative to traditional amperometric-based glucometry.