Autor segons l'article: Patraca I, Martínez N, Busquets O, Martí A, Pedrós I, Beas-Zarate C, Marin M, Ettcheto M, Sureda F, Auladell C, Camins A, Folch J.
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: PATRACA FIERRO, JOSÉ IVÁN; MARTINEZ LOPEZ, NOHORA MILENA; Martí, A.; Pedrós, I.; Beas-Zarate, C.; Marin, M.; Ettcheto, M.; Sureda, F.; Auladell, C.; Camins, A.; FOLCH LOPEZ, JAUME
Paraules clau: Leptin
Resum: Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.
Grup de recerca: Farmacobiologia Cel.lular
Àrees temàtiques: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1734-1140
Data d'alta del registre: 2017-03-14
Pàgina final: 418
Volum de revista: 69
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1016/j.pharep.2016.12.005
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2017
Pàgina inicial: 409
Tipus de publicació: Article Artículo Article
Repositori URV