Articles producció científica> Bioquímica i Biotecnologia

Role of Mitochondrial Retrograde Pathway in Regulating Ethanol-Inducible Filamentous Growth in Yeast

  • Dades identificatives

    Identificador: PC:2804
    Autors:
    Mas, A.González, B.Beltran, G.Cullen, P.J.Torija, M.J.
    Resum:
    In yeast, ethanol is produced as a by-product of fermentation through glycolysis. Ethanol also stimulates a developmental foraging response called filamentous growth and is thought to act as a quorum-sensing molecule. Ethanol-inducible filamentous growth was examined in a small collection of wine/European strains, which validated ethanol as an inducer of filamentous growth. Wine strains also showed variability in their filamentation responses, which illustrates the striking phenotypic differences that can occur among individuals. Ethanol-inducible filamentous growth in 61278b strains was independent of several of the major filamentation regulatory pathways [including fMAPK, RAS-cAMP, Snf1, Rpd3(L), and Rim101] but required the mitochondrial retrograde (RTG) pathway, an inter-organellar signaling pathway that controls the nuclear response to defects in mitochondrial function. The RTG pathway regulated ethanol-dependent filamentous growth by maintaining flux through the TCA cycle. The ethanol-dependent invasive growth response required the polarisome and transcriptional induction of the cell adhesion molecule Flo11p. Our results validate established stimuli that trigger filamentous growth and show how stimuli can trigger highly specific responses among individuals. Our results also connect an inter-organellar pathway to a quorum sensing response in fungi.
  • Altres:

    Autor segons l'article: Mas, A.; González, B.; Beltran, G.; Cullen, P.J.; Torija, M.J.
    Departament: Bioquímica i Biotecnologia
    Autor/s de la URV: MAS BARON, ALBERTO; González, B.; BELTRAN CASELLAS, GEMMA; Cullen, P.J.; TORIJA MARTÍNEZ, MARÍA JESÚS
    Paraules clau: filamentous growth pseudohyphal growth quorum sensing
    Resum: In yeast, ethanol is produced as a by-product of fermentation through glycolysis. Ethanol also stimulates a developmental foraging response called filamentous growth and is thought to act as a quorum-sensing molecule. Ethanol-inducible filamentous growth was examined in a small collection of wine/European strains, which validated ethanol as an inducer of filamentous growth. Wine strains also showed variability in their filamentation responses, which illustrates the striking phenotypic differences that can occur among individuals. Ethanol-inducible filamentous growth in 61278b strains was independent of several of the major filamentation regulatory pathways [including fMAPK, RAS-cAMP, Snf1, Rpd3(L), and Rim101] but required the mitochondrial retrograde (RTG) pathway, an inter-organellar signaling pathway that controls the nuclear response to defects in mitochondrial function. The RTG pathway regulated ethanol-dependent filamentous growth by maintaining flux through the TCA cycle. The ethanol-dependent invasive growth response required the polarisome and transcriptional induction of the cell adhesion molecule Flo11p. Our results validate established stimuli that trigger filamentous growth and show how stimuli can trigger highly specific responses among individuals. Our results also connect an inter-organellar pathway to a quorum sensing response in fungi.
    Grup de recerca: Biotecnologia Enològica
    Àrees temàtiques: Enologia Enología Oenology
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1664-042X
    Identificador de l'autor: 0000-0002-0763-1679; n/a; 0000-0002-7071-205X; n/a; 0000-0001-6419-0745
    Data d'alta del registre: 2017-05-18
    Volum de revista: 8
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: http://journal.frontiersin.org/article/10.3389/fphys.2017.00148/full
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.3389/fphys.2017.00148
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2017
    Pàgina inicial: Art.num. 148
    Tipus de publicació: Article Artículo Article
  • Paraules clau:

    Llevats
    Mitocondris
    filamentous growth
    pseudohyphal growth
    quorum sensing
    Enologia
    Enología
    Oenology
    1664-042X
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