Articles producció científica> Química Analítica i Química Orgànica

A disposable, simple, fast and low-cost paper-based biosensor and its application to the determination of glucose in commercial orange juices

  • Dades identificatives

    Identificador: PC:3322
    Autors:
    Novell, M.Guadarrama-Fernández, L.Blondeau, P.Andrade, F. J.
    Resum:
    A new biosensor for monitoring glucose levels in beverages is presented. The measurements are performed using potentiometric detection. Working electrodes are made using platinised paper as support and a biocompatible polymeric membrane made of a mixture of polyvinyl alcohol and chitosan containing glucose oxidase as the recognition layer. The system is based on the detection of the hydrogen peroxide generated by an enzymatic reaction performed in a highly sensitive, selective and simple way. The biosensors display suitable analytical performance (sensitivity −119.6 ± 6.4 mV/dec in the 0.03–1.0 mM range with a limit of detection of 0.02 mM). Determination of glucose in commercial orange juices is presented. These results were validated against conventional standard methods, showing good accuracy and fast analytical response. The methodology presented herein does not require complex samples treatment, offering an alternative to conventional methods, particularly for determinations performed with minimal expertise and without a laboratory infrastructure.
  • Altres:

    Autor segons l'article: Novell, M.; Guadarrama-Fernández, L.; Blondeau, P.; Andrade, F. J.
    Departament: Química Analítica i Química Orgànica
    Autor/s de la URV: NOVELL RECASENS, MARTA; Guadarrama-Fernández, L.; BLONDEAU , PASCAL JEAN CLAUDE LEON; ANDRADE , FRANCISCO JAVIER
    Resum: A new biosensor for monitoring glucose levels in beverages is presented. The measurements are performed using potentiometric detection. Working electrodes are made using platinised paper as support and a biocompatible polymeric membrane made of a mixture of polyvinyl alcohol and chitosan containing glucose oxidase as the recognition layer. The system is based on the detection of the hydrogen peroxide generated by an enzymatic reaction performed in a highly sensitive, selective and simple way. The biosensors display suitable analytical performance (sensitivity −119.6 ± 6.4 mV/dec in the 0.03–1.0 mM range with a limit of detection of 0.02 mM). Determination of glucose in commercial orange juices is presented. These results were validated against conventional standard methods, showing good accuracy and fast analytical response. The methodology presented herein does not require complex samples treatment, offering an alternative to conventional methods, particularly for determinations performed with minimal expertise and without a laboratory infrastructure.
    Grup de recerca: Grup de Quimiometria, Qualimetria i Nanosensors
    Àrees temàtiques: Química Química Chemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0308-8146
    Identificador de l'autor: ; ; 0000-0003-1331-5055; 0000-0003-1199-2837
    Data d'alta del registre: 2018-07-27
    Pàgina final: 69
    Volum de revista: 265
    Versió de l'article dipositat: info:eu-repo/semantics/submittedVersion
    Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S030881461830894X?via%3Dihub
    Programa de finançament: plan; Retos; CTQ2013-46404-R plan; Retos; CTQ2016-77128-R altres; Ramon y Cajal ; RyC; altres; CONACyT (Mexico) post doctorate scholarship;
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.1016/j.foodchem.2018.05.082
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2018
    Pàgina inicial: 64
    Tipus de publicació: Article Artículo Article
  • Paraules clau:

    Biosensors
    Glucosa
    Suc de taronja
    Química
    Química
    Chemistry
    0308-8146
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