Articles producció científica> Química Analítica i Química Orgànica

A disposable, simple, fast and low-cost paper-based biosensor and its application to the determination of glucose in commercial orange juices

  • Datos identificativos

    Identificador: PC:3322
    Autores:
    Novell, M.Guadarrama-Fernández, L.Blondeau, P.Andrade, F. J.
    Resumen:
    A new biosensor for monitoring glucose levels in beverages is presented. The measurements are performed using potentiometric detection. Working electrodes are made using platinised paper as support and a biocompatible polymeric membrane made of a mixture of polyvinyl alcohol and chitosan containing glucose oxidase as the recognition layer. The system is based on the detection of the hydrogen peroxide generated by an enzymatic reaction performed in a highly sensitive, selective and simple way. The biosensors display suitable analytical performance (sensitivity −119.6 ± 6.4 mV/dec in the 0.03–1.0 mM range with a limit of detection of 0.02 mM). Determination of glucose in commercial orange juices is presented. These results were validated against conventional standard methods, showing good accuracy and fast analytical response. The methodology presented herein does not require complex samples treatment, offering an alternative to conventional methods, particularly for determinations performed with minimal expertise and without a laboratory infrastructure.
  • Otros:

    Autor según el artículo: Novell, M.; Guadarrama-Fernández, L.; Blondeau, P.; Andrade, F. J.
    Departamento: Química Analítica i Química Orgànica
    Autor/es de la URV: NOVELL RECASENS, MARTA; Guadarrama-Fernández, L.; BLONDEAU , PASCAL JEAN CLAUDE LEON; ANDRADE , FRANCISCO JAVIER
    Resumen: A new biosensor for monitoring glucose levels in beverages is presented. The measurements are performed using potentiometric detection. Working electrodes are made using platinised paper as support and a biocompatible polymeric membrane made of a mixture of polyvinyl alcohol and chitosan containing glucose oxidase as the recognition layer. The system is based on the detection of the hydrogen peroxide generated by an enzymatic reaction performed in a highly sensitive, selective and simple way. The biosensors display suitable analytical performance (sensitivity −119.6 ± 6.4 mV/dec in the 0.03–1.0 mM range with a limit of detection of 0.02 mM). Determination of glucose in commercial orange juices is presented. These results were validated against conventional standard methods, showing good accuracy and fast analytical response. The methodology presented herein does not require complex samples treatment, offering an alternative to conventional methods, particularly for determinations performed with minimal expertise and without a laboratory infrastructure.
    Grupo de investigación: Grup de Quimiometria, Qualimetria i Nanosensors
    Áreas temáticas: Química Química Chemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0308-8146
    Identificador del autor: ; ; 0000-0003-1331-5055; 0000-0003-1199-2837
    Fecha de alta del registro: 2018-07-27
    Página final: 69
    Volumen de revista: 265
    Versión del articulo depositado: info:eu-repo/semantics/submittedVersion
    Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S030881461830894X?via%3Dihub
    Programa de financiación: plan; Retos; CTQ2013-46404-R plan; Retos; CTQ2016-77128-R altres; Ramon y Cajal ; RyC; altres; CONACyT (Mexico) post doctorate scholarship;
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.1016/j.foodchem.2018.05.082
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2018
    Página inicial: 64
    Tipo de publicación: Article Artículo Article
  • Palabras clave:

    Biosensors
    Glucosa
    Suc de taronja
    Química
    Química
    Chemistry
    0308-8146
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