Articles producció científica> Química Analítica i Química Orgànica

A disposable, simple, fast and low-cost paper-based biosensor and its application to the determination of glucose in commercial orange juices

  • Identification data

    Identifier: PC:3322
    Authors:
    Novell, M.Guadarrama-Fernández, L.Blondeau, P.Andrade, F. J.
    Abstract:
    A new biosensor for monitoring glucose levels in beverages is presented. The measurements are performed using potentiometric detection. Working electrodes are made using platinised paper as support and a biocompatible polymeric membrane made of a mixture of polyvinyl alcohol and chitosan containing glucose oxidase as the recognition layer. The system is based on the detection of the hydrogen peroxide generated by an enzymatic reaction performed in a highly sensitive, selective and simple way. The biosensors display suitable analytical performance (sensitivity −119.6 ± 6.4 mV/dec in the 0.03–1.0 mM range with a limit of detection of 0.02 mM). Determination of glucose in commercial orange juices is presented. These results were validated against conventional standard methods, showing good accuracy and fast analytical response. The methodology presented herein does not require complex samples treatment, offering an alternative to conventional methods, particularly for determinations performed with minimal expertise and without a laboratory infrastructure.
  • Others:

    Author, as appears in the article.: Novell, M.; Guadarrama-Fernández, L.; Blondeau, P.; Andrade, F. J.
    Department: Química Analítica i Química Orgànica
    URV's Author/s: NOVELL RECASENS, MARTA; Guadarrama-Fernández, L.; BLONDEAU , PASCAL JEAN CLAUDE LEON; ANDRADE , FRANCISCO JAVIER
    Abstract: A new biosensor for monitoring glucose levels in beverages is presented. The measurements are performed using potentiometric detection. Working electrodes are made using platinised paper as support and a biocompatible polymeric membrane made of a mixture of polyvinyl alcohol and chitosan containing glucose oxidase as the recognition layer. The system is based on the detection of the hydrogen peroxide generated by an enzymatic reaction performed in a highly sensitive, selective and simple way. The biosensors display suitable analytical performance (sensitivity −119.6 ± 6.4 mV/dec in the 0.03–1.0 mM range with a limit of detection of 0.02 mM). Determination of glucose in commercial orange juices is presented. These results were validated against conventional standard methods, showing good accuracy and fast analytical response. The methodology presented herein does not require complex samples treatment, offering an alternative to conventional methods, particularly for determinations performed with minimal expertise and without a laboratory infrastructure.
    Research group: Grup de Quimiometria, Qualimetria i Nanosensors
    Thematic Areas: Química Química Chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0308-8146
    Author identifier: ; ; 0000-0003-1331-5055; 0000-0003-1199-2837
    Record's date: 2018-07-27
    Last page: 69
    Journal volume: 265
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S030881461830894X?via%3Dihub
    Funding program: plan; Retos; CTQ2013-46404-R plan; Retos; CTQ2016-77128-R altres; Ramon y Cajal ; RyC; altres; CONACyT (Mexico) post doctorate scholarship;
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.foodchem.2018.05.082
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    First page: 64
    Publication Type: Article Artículo Article
  • Keywords:

    Biosensors
    Glucosa
    Suc de taronja
    Química
    Química
    Chemistry
    0308-8146
  • Documents:

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