Autor segons l'article: Fernández-Castillejo, Sara; Yuste, Sílvia; Macià, Alba; Ludwig, Izíar; Romero, María-Paz; Catalán, Úrsula; Motilva, Maria-José; Rubió, Laura
Departament: Medicina i Cirurgia
Autor/s de la URV: FERNÁNDEZ CASTILLEJO, SARA; Yuste, Sílvia; Macià, Alba; Ludwig, Izíar; Romero, María-Paz; CATALÁN SANTOS, ÚRSULA; Motilva, Maria-José; Rubió, Laura
Paraules clau: phenolic metabolites Plasma samples
Resum: SCOPE:
The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied.
METHODS AND RESULTS:
An analytical method based on blood sampling with DBS cards combined with LC-MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red-fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (µSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl-γ-valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or µSPE.
CONCLUSIONS:
Finger-prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.
Grup de recerca: Unitat de Recerca de Lípids i Arteriosclerosi
Àrees temàtiques: Health sciences Ciencias de la salud Ciències de la salut
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1613-4125
Identificador de l'autor: 0000-0002-8109-9713; ; ; ; ; 0000-0001-8884-9823; ;
Data d'alta del registre: 2019-03-22
Volum de revista: 62
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2018
Pàgina inicial: 1800623
Tipus de publicació: Article Artículo Article
Repositori URV