Author, as appears in the article.: Fernández-Castillejo, Sara; Yuste, Sílvia; Macià, Alba; Ludwig, Izíar; Romero, María-Paz; Catalán, Úrsula; Motilva, Maria-José; Rubió, Laura
Department: Medicina i Cirurgia
URV's Author/s: FERNÁNDEZ CASTILLEJO, SARA; Yuste, Sílvia; Macià, Alba; Ludwig, Izíar; Romero, María-Paz; CATALÁN SANTOS, ÚRSULA; Motilva, Maria-José; Rubió, Laura
Keywords: phenolic metabolites Plasma samples
Abstract: SCOPE:
The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied.
METHODS AND RESULTS:
An analytical method based on blood sampling with DBS cards combined with LC-MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red-fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (µSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl-γ-valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or µSPE.
CONCLUSIONS:
Finger-prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.
Research group: Unitat de Recerca de Lípids i Arteriosclerosi
Thematic Areas: Health sciences Ciencias de la salud Ciències de la salut
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1613-4125
Author identifier: 0000-0002-8109-9713; ; ; ; ; 0000-0001-8884-9823; ;
Record's date: 2019-03-22
Journal volume: 62
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201800623
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1002/mnfr.201800623
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
First page: 1800623
Publication Type: Article Artículo Article