Articles producció científica> Enginyeria Química

Cell-based assay coupled with chromatographic fractioning: A strategy for marine toxins detection in natural samples

  • Dades identificatives

    Identificador: imarina:3190404
    Autors:
    Diogene, J.Gimenez, G.de la Iglesia, P.Canete, E.Caillaud, A.
    Resum:
    Cell-based assays (CBA) have been proposed for the evaluation of toxicity caused by marine toxins in natural samples (fish, shellfish and microalgae). However, their application has been hindered due to the interferences present in biological matrices that may cause cellular response and interfere in toxicity evaluation. This work reviews in an extensive introduction the use of CBA for toxicity evaluation of marine toxins. Afterwards, the coupling of chromatographic fractioning with neuroblastoma Neuro-2a CBA is presented to enhance the applicability of CBA for complex matrices. Examples of application are provided for mussel samples (Mytilus galloprovincialis) and microalgae (Gambierdiscus sp.), and the results demonstrated the great potential of the combined strategy for reliable toxicological evaluation without ethical concern. Fractioning of an equivalent of 72 mg eq mL(-1) of mussel sample allowed the identification of non-toxic and toxic fractions whereas only 2.5mg eq mL(-1) of non-purified mussel sample was responsible for 20% of cell mortality. Furthermore, the application of CBA allowed selectively distinguishing between ciguatoxin-like and other unspecific toxicity in Gambierdiscus sp. extract.
  • Altres:

    Autor segons l'article: Diogene, J. Gimenez, G. de la Iglesia, P. Canete, E. Caillaud, A.
    Departament: REVISAR - Enginyeria Química
    Autor/s de la URV: GIMÉNEZ PAPIOL, GEMMA
    Paraules clau: SHELLFISH Neuroblastoma Microalgae Marine toxins Gambierdiscus Fractioning Fish Cytotoxicity Cell-Based Assay
    Resum: Cell-based assays (CBA) have been proposed for the evaluation of toxicity caused by marine toxins in natural samples (fish, shellfish and microalgae). However, their application has been hindered due to the interferences present in biological matrices that may cause cellular response and interfere in toxicity evaluation. This work reviews in an extensive introduction the use of CBA for toxicity evaluation of marine toxins. Afterwards, the coupling of chromatographic fractioning with neuroblastoma Neuro-2a CBA is presented to enhance the applicability of CBA for complex matrices. Examples of application are provided for mussel samples (Mytilus galloprovincialis) and microalgae (Gambierdiscus sp.), and the results demonstrated the great potential of the combined strategy for reliable toxicological evaluation without ethical concern. Fractioning of an equivalent of 72 mg eq mL(-1) of mussel sample allowed the identification of non-toxic and toxic fractions whereas only 2.5mg eq mL(-1) of non-purified mussel sample was responsible for 20% of cell mortality. Furthermore, the application of CBA allowed selectively distinguishing between ciguatoxin-like and other unspecific toxicity in Gambierdiscus sp. extract.
    Àrees temàtiques: Toxicology Medicine (Miscellaneous)
    Adreça de correu electrònic de l'autor: gemma.gimenez@urv.cat
    ISSN: 08872333
    Data d'alta del registre: 2020-01-29
    Referència a l'article segons font original: TOXICOLOGY IN VITRO. (ISSN/ISBN: 08872333). 23(8): 1591-1596
    Referència de l'ítem segons les normes APA: Caillaud, A.; Canete, E.; de la Iglesia, P.; Gimenez, G.; Diogene, J. (2009). Cell-based assay coupled with chromatographic fractioning: A strategy for marine toxins detection in natural samples. TOXICOLOGY IN VITRO, 23(8), 1591-1596. DOI: 10.1016/j.tiv.2009.08.013
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.1016/j.tiv.2009.08.013
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2009
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Medicine (Miscellaneous),Toxicology,Transplantation
    SHELLFISH
    Neuroblastoma
    Microalgae
    Marine toxins
    Gambierdiscus
    Fractioning
    Fish
    Cytotoxicity
    Cell-Based Assay
    Toxicology
    Medicine (Miscellaneous)
    08872333
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