Articles producció científica> Enginyeria Química

The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells

  • Dades identificatives

    Identificador: imarina:5131851
    Autors:
    Orlowska A, Perera PT, Al Kobaisi MA, Dias A, Nguyen HKD, Ghanaati S, Baulin V, Crawford RJ, Ivanova EP
    Resum:
    Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied. Cell morphology was visualised using brightfield phase contrast microscopy, cellular metabolism and proliferation were quantified using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, and the neurite outgrowth and axonal generation of the PC12 cells were evaluated using wide field fluorescence microscopy. It was found that double coatings of PLL/Lam and PLL/Fn supported robust adhesion and a two-fold enhanced neurite outgrowth of PC12 cells when treated with 100 ng/mL of NGF while exhibiting stable metabolic activity, leading to the accelerated generation of axons.
  • Altres:

    Autor segons l'article: Orlowska A, Perera PT, Al Kobaisi MA, Dias A, Nguyen HKD, Ghanaati S, Baulin V, Crawford RJ, Ivanova EP
    Departament: Enginyeria Química
    Autor/s de la URV: Baulin, Vladimir
    Paraules clau: Substratum Proliferation Poly-l-lysine Pc12 neuronal differentiation Neuronal differentiation Nerve growth factor (ngf) Laminin Fibronectin Adhesion pc12 neuronal differentiation nerve growth factor (ngf) laminin fibronectin
    Resum: Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied. Cell morphology was visualised using brightfield phase contrast microscopy, cellular metabolism and proliferation were quantified using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, and the neurite outgrowth and axonal generation of the PC12 cells were evaluated using wide field fluorescence microscopy. It was found that double coatings of PLL/Lam and PLL/Fn supported robust adhesion and a two-fold enhanced neurite outgrowth of PC12 cells when treated with 100 ng/mL of NGF while exhibiting stable metabolic activity, leading to the accelerated generation of axons.
    Àrees temàtiques: Química Odontología Medicina veterinaria Medicina i Materials science, multidisciplinary Materiais Interdisciplinar General materials science Engenharias iv Engenharias iii Engenharias ii Engenharias i Condensed matter physics Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Astronomia / física Arquitetura, urbanismo e design
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 19961944
    Adreça de correu electrònic de l'autor: vladimir.baulin@urv.cat
    Identificador de l'autor: 0000-0003-2086-4271
    Data d'alta del registre: 2023-07-31
    Volum de revista: 11
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/1996-1944/11/1/60
    Referència a l'article segons font original: Materials. 11 (1): 60-
    Referència de l'ítem segons les normes APA: Orlowska A, Perera PT, Al Kobaisi MA, Dias A, Nguyen HKD, Ghanaati S, Baulin V, Crawford RJ, Ivanova EP (2018). The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells. Materials, 11(1), 60-. DOI: 10.3390/ma11010060
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.3390/ma11010060
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2018
    Pàgina inicial: Article number 60
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Materials Science, Multidisciplinary
    Substratum
    Proliferation
    Poly-l-lysine
    Pc12 neuronal differentiation
    Neuronal differentiation
    Nerve growth factor (ngf)
    Laminin
    Fibronectin
    Adhesion
    pc12 neuronal differentiation
    nerve growth factor (ngf)
    laminin
    fibronectin
    Química
    Odontología
    Medicina veterinaria
    Medicina i
    Materials science, multidisciplinary
    Materiais
    Interdisciplinar
    General materials science
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Condensed matter physics
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Astronomia / física
    Arquitetura, urbanismo e design
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