Articles producció científica> Ciències Mèdiques Bàsiques

Understanding Mucor circinelloides pathogenesis by comparative genomics and phenotypical studies

  • Dades identificatives

    Identificador: imarina:5132650
    Autors:
    López-Fernández L., Sanchis M., Navarro-Rodríguez P., Nicolás F., Silva-Franco F., Guarro J., Garre V., Navarro-Mendoza M., Pérez-Arques C., Capilla J.
    Resum:
    The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. This study aims to decipher the genetic basis of Mucor circinelloides pathogenicity, which displays virulence in a strain dependent manner. Assuming that genetic differences between strains may be linked to different pathotypes, we have conducted a study to explore genes responsible for virulence in M. circinelloides by whole genome sequencing of the avirulent strain NRRL3631 and comparison with the virulent strain CBS277.49. This genome analysis revealed 773 truncated, discontiguous and absent genes in the NRRL3631 strain. We also examined phenotypic traits resulting in reduced heat stress tolerance, chitosan content and lower susceptibility to toxic compounds (calcofluor white and sodium dodecyl sulphate) in the virulent strain, suggesting the influence of cell wall on pathogenesis. Based on these results, we focused on studying extracellular protein-coding genes by gene deletion and further pathotype characterization of mutants in murine models of pulmonary and systemic infection. Deletion of gene ID112092, which codes for a hypothetical extracellular protein of unknown function, resulted in significant reduction of virulence. Although pathogenesis is a multifactorial process, these findings highlight the crucial role of surface and secreted proteins in M. circinelloides virulence and should promote further studies of other differential genes.
  • Altres:

    Autor segons l'article: López-Fernández L., Sanchis M., Navarro-Rodríguez P., Nicolás F., Silva-Franco F., Guarro J., Garre V., Navarro-Mendoza M., Pérez-Arques C., Capilla J.
    Departament: Ciències Mèdiques Bàsiques
    Autor/s de la URV: Capilla Luque, Javier / Guarro Artigas, Josep / Sanchis Talón, Marta
    Paraules clau: Whole genome sequencing Virulence factors Virulence factor Proteins Murine model infection Mucormycosis Mucor Genome comparison Gene Fungi Fungal pathogenesis Chitosan Chitin Cell-wall Aspergillus-fumigatus virulence factors virulence factor murine model infection mucormycosis mucor genome comparison fungal pathogenesis
    Resum: The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. This study aims to decipher the genetic basis of Mucor circinelloides pathogenicity, which displays virulence in a strain dependent manner. Assuming that genetic differences between strains may be linked to different pathotypes, we have conducted a study to explore genes responsible for virulence in M. circinelloides by whole genome sequencing of the avirulent strain NRRL3631 and comparison with the virulent strain CBS277.49. This genome analysis revealed 773 truncated, discontiguous and absent genes in the NRRL3631 strain. We also examined phenotypic traits resulting in reduced heat stress tolerance, chitosan content and lower susceptibility to toxic compounds (calcofluor white and sodium dodecyl sulphate) in the virulent strain, suggesting the influence of cell wall on pathogenesis. Based on these results, we focused on studying extracellular protein-coding genes by gene deletion and further pathotype characterization of mutants in murine models of pulmonary and systemic infection. Deletion of gene ID112092, which codes for a hypothetical extracellular protein of unknown function, resulted in significant reduction of virulence. Although pathogenesis is a multifactorial process, these findings highlight the crucial role of surface and secreted proteins in M. circinelloides virulence and should promote further studies of other differential genes.
    Àrees temàtiques: Saúde coletiva Parasitology Odontología Microbiology (medical) Microbiology Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology General medicine Farmacia Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 21505594
    Adreça de correu electrònic de l'autor: marta.sanchis@urv.cat javier.capilla@urv.cat
    Identificador de l'autor: 0000-0001-7251-6792 0000-0002-0765-6403
    Data d'alta del registre: 2024-09-07
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.tandfonline.com/doi/full/10.1080/21505594.2018.1435249
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Virulence. 9 (1): 707-720
    Referència de l'ítem segons les normes APA: López-Fernández L., Sanchis M., Navarro-Rodríguez P., Nicolás F., Silva-Franco F., Guarro J., Garre V., Navarro-Mendoza M., Pérez-Arques C., Capilla J (2018). Understanding Mucor circinelloides pathogenesis by comparative genomics and phenotypical studies. Virulence, 9(1), 707-720. DOI: 10.1080/21505594.2018.1435249
    DOI de l'article: 10.1080/21505594.2018.1435249
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2018
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Immunology,Infectious Diseases,Microbiology,Microbiology (Medical),Parasitology
    Whole genome sequencing
    Virulence factors
    Virulence factor
    Proteins
    Murine model infection
    Mucormycosis
    Mucor
    Genome comparison
    Gene
    Fungi
    Fungal pathogenesis
    Chitosan
    Chitin
    Cell-wall
    Aspergillus-fumigatus
    virulence factors
    virulence factor
    murine model infection
    mucormycosis
    mucor
    genome comparison
    fungal pathogenesis
    Saúde coletiva
    Parasitology
    Odontología
    Microbiology (medical)
    Microbiology
    Medicina ii
    Medicina i
    Interdisciplinar
    Infectious diseases
    Immunology
    General medicine
    Farmacia
    Engenharias i
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciência de alimentos
    Biotecnología
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