Autor según el artículo: López-Fernández L., Sanchis M., Navarro-Rodríguez P., Nicolás F., Silva-Franco F., Guarro J., Garre V., Navarro-Mendoza M., Pérez-Arques C., Capilla J.

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: Capilla Luque, Javier / Guarro Artigas, Josep / Sanchis Talón, Marta

Palabras clave: Whole genome sequencing Virulence factors Virulence factor Proteins Murine model infection Mucormycosis Mucor Genome comparison Gene Fungi Fungal pathogenesis Chitosan Chitin Cell-wall Aspergillus-fumigatus virulence factors virulence factor murine model infection mucormycosis mucor genome comparison fungal pathogenesis

Resumen: The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. This study aims to decipher the genetic basis of Mucor circinelloides pathogenicity, which displays virulence in a strain dependent manner. Assuming that genetic differences between strains may be linked to different pathotypes, we have conducted a study to explore genes responsible for virulence in M. circinelloides by whole genome sequencing of the avirulent strain NRRL3631 and comparison with the virulent strain CBS277.49. This genome analysis revealed 773 truncated, discontiguous and absent genes in the NRRL3631 strain. We also examined phenotypic traits resulting in reduced heat stress tolerance, chitosan content and lower susceptibility to toxic compounds (calcofluor white and sodium dodecyl sulphate) in the virulent strain, suggesting the influence of cell wall on pathogenesis. Based on these results, we focused on studying extracellular protein-coding genes by gene deletion and further pathotype characterization of mutants in murine models of pulmonary and systemic infection. Deletion of gene ID112092, which codes for a hypothetical extracellular protein of unknown function, resulted in significant reduction of virulence. Although pathogenesis is a multifactorial process, these findings highlight the crucial role of surface and secreted proteins in M. circinelloides virulence and should promote further studies of other differential genes.

Áreas temáticas: Saúde coletiva Parasitology Odontología Microbiology (medical) Microbiology Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology General medicine Farmacia Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 21505594

Direcció de correo del autor: marta.sanchis@urv.cat josep.guarro@urv.cat javier.capilla@urv.cat

Identificador del autor: 0000-0001-7251-6792 0000-0002-7839-7568 0000-0002-0765-6403

Fecha de alta del registro: 2023-02-18

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.tandfonline.com/doi/full/10.1080/21505594.2018.1435249

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Virulence. 9 (1): 707-720

Referencia de l'ítem segons les normes APA: López-Fernández L., Sanchis M., Navarro-Rodríguez P., Nicolás F., Silva-Franco F., Guarro J., Garre V., Navarro-Mendoza M., Pérez-Arques C., Capilla J (2018). Understanding Mucor circinelloides pathogenesis by comparative genomics and phenotypical studies. Virulence, 9(1), 707-720. DOI: 10.1080/21505594.2018.1435249

DOI del artículo: 10.1080/21505594.2018.1435249

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2018

Tipo de publicación: Journal Publications