Dual DNA barcoding for the molecular identification of the agents of invasive fungal infections

Autor segons l'article: Hoang MTV, Irinyi L, Chen SCA, Sorrell TC,, Meyer W ISHAM Barcoding of Medical Fungi Working Group

Departament: Ciències Mèdiques Bàsiques

Autor/s de la URV: Cano Lira, José Francisco / Guarro Artigas, Josep

Paraules clau: Translational elongation factor 1α; Translational elongation factor 1?; Isham barcoding database; Invasive fungal diseases; Internal transcribed spacer region; Identification; Fungal dna barcoding; Dual barcoding system; isham barcoding database; invasive fungal diseases; internal transcribed spacer region; identification; fungal dna barcoding; dual barcoding system

Resum: Copyright © 2019 Hoang, Irinyi, Chen, Sorrell, the ISHAM Barcoding of Medical Fungi Working Group and Meyer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Invasive fungal infections, such as aspergillosis, candidiasis, and cryptococcosis, have significantly increased among immunocompromised people. To tackle these infections the first and most decisive step is the accurate identification of the causal pathogen. Routine identification of invasive fungal infections has progressed away from culture-dependent methods toward molecular techniques, including DNA barcoding, a highly efficient and widely used diagnostic technique. Fungal DNA barcoding previously relied on a single barcoding region, the internal transcribed spacer (ITS) region. However, this allowed only for 75% of all fungi to be correctly identified. As such, the translational elongation factor 1α (TEF1α) was recently introduced as the secondary barcode region to close the gap. Both loci together form the dual fungal DNA barcoding scheme. As a result, the ISHAM Barcoding Database has been expanded to include sequences for both barcoding regions to enable practical implementation of the dual barcoding scheme into clinical practice. The present study investigates the impact of the secondary barcode on the identification of clinically important fungal taxa, that have been demonstrated to cause severe invasive disease. Analysis of the barcoding regions was performed using barcoding gap analysis based on the genetic distances generated with the Kimura 2-parameter model. The secondary barcode demonstrated an improvement in identification for all taxa that were unidentifiable with the primary barcode, and when combined with the primary barcode ensured accurate identification for all taxa analyzed, making DNA barcoding an important, efficient and reliable addition to the diagnostic toolset of invasive fungal infections.

Àrees temàtiques: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Odontología; Nutrição; Microbiology (medical); Microbiology; Medicina veterinaria; Medicina ii; Medicina i; Materiais; Matemática / probabilidade e estatística; Interdisciplinar; Geografía; Geociências; Farmacia; Ensino; Engenharias iii; Engenharias ii; Engenharias i; Economia; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência de alimentos; Ciência da computação; Biotecnología; Biodiversidade; Astronomia / física

Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1664302X

Adreça de correu electrònic de l'autor: jose.cano@urv.cat

Data d'alta del registre: 2025-01-28

Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion

Enllaç font original: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01647/full

URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/

Referència a l'article segons font original: Frontiers In Microbiology. 10 (JULY): 1647-

Referència de l'ítem segons les normes APA: Hoang MTV, Irinyi L, Chen SCA, Sorrell TC,, Meyer W ISHAM Barcoding of Medical Fungi Working Group (2019). Dual DNA barcoding for the molecular identification of the agents of invasive fungal infections. Frontiers In Microbiology, 10(JULY), 1647-. DOI: 10.3389/fmicb.2019.01647

DOI de l'article: 10.3389/fmicb.2019.01647

Entitat: Universitat Rovira i Virgili

Any de publicació de la revista: 2019

Tipus de publicació: Journal Publications