Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection

Autor segons l'article: Pol, Laura; Karen Acosta, Laura; Ferre-Borrull, Josep; Marsal, Lluis F

Departament: Enginyeria Electrònica, Elèctrica i Automàtica

e-ISSN: 1424-8220

Autor/s de la URV: Acosta Capilla, Laura Karen / Ferré Borrull, José / Marsal Garví, Luis Francisco / POL AIRA, LAURA

Paraules clau: Thrombin aptamer; Thrombin; Streptavidin; Selection; Rifs; Perspective; Nanoporous anodic alumina; Nanopores; Molecules; Limit of detection; Interferometry; Electrodes; Electrochemical detection; Biosensing techniques; Biosensing; Bind; Aptamers, nucleotide; Aptamers; Aluminum oxide; streptavidin; rifs; nanoporous anodic alumina; biosensing; aptamers

Resum: Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient manner. These chemical modifications must be well defined. In this work we propose nanoporous anodic alumina (NAA) chemically modified with streptavidin as a platform for aptamer immobilization. The immobilization of biotinylated thrombin binding aptamer (TBA) was monitored in real time by means of reflective interferometric spectroscopy (RIfS). The study has permitted to characterize in real time the path to immobilize TBA on the inner pore walls of NAA. Furthermore, this study provides an accurate label-free method to detect thrombin in real-time with high affinity and specificity.

Àrees temàtiques: Zootecnia / recursos pesqueiros; Química; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Materiais; Matemática / probabilidade e estatística; Linguística e literatura; Letras / linguística; Interdisciplinar; Instruments & instrumentation; Instrumentation; Information systems; Geografía; Geociências; Farmacia; Engineering, electrical & electronic; Engenharias iv; Engenharias iii; Engenharias ii; Engenharias i; Electrochemistry; Electrical and electronic engineering; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência de alimentos; Ciência da computação; Chemistry, analytical; Biotecnología; Biodiversidade; Biochemistry; Atomic and molecular physics, and optics; Astronomia / física; Arquitetura, urbanismo e design; Analytical chemistry

Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14248220

Adreça de correu electrònic de l'autor: laurakaren.acosta@urv.cat; josep.ferre@urv.cat; lluis.marsal@urv.cat

Data d'alta del registre: 2024-10-12

Volum de revista: 19

Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion

Enllaç font original: https://www.mdpi.com/1424-8220/19/20/4543

URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/

Referència a l'article segons font original: Sensors. 19 (20): 4543-

Referència de l'ítem segons les normes APA: Pol, Laura; Karen Acosta, Laura; Ferre-Borrull, Josep; Marsal, Lluis F (2019). Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection. Sensors, 19(20), 4543-. DOI: 10.3390/s19204543

DOI de l'article: 10.3390/s19204543

Entitat: Universitat Rovira i Virgili

Any de publicació de la revista: 2019

Tipus de publicació: Journal Publications