Author, as appears in the article.: Pol, Laura; Karen Acosta, Laura; Ferre-Borrull, Josep; Marsal, Lluis F
Department: Enginyeria Electrònica, Elèctrica i Automàtica
e-ISSN: 1424-8220
URV's Author/s: Acosta Capilla, Laura Karen / Ferré Borrull, José / Marsal Garví, Luis Francisco / POL AIRA, LAURA
Keywords: Thrombin aptamer Thrombin Streptavidin Selection Rifs Perspective Nanoporous anodic alumina Nanopores Molecules Limit of detection Interferometry Electrodes Electrochemical detection Biosensing techniques Biosensing Bind Aptamers, nucleotide Aptamers Aluminum oxide streptavidin rifs nanoporous anodic alumina biosensing aptamers
Abstract: Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient manner. These chemical modifications must be well defined. In this work we propose nanoporous anodic alumina (NAA) chemically modified with streptavidin as a platform for aptamer immobilization. The immobilization of biotinylated thrombin binding aptamer (TBA) was monitored in real time by means of reflective interferometric spectroscopy (RIfS). The study has permitted to characterize in real time the path to immobilize TBA on the inner pore walls of NAA. Furthermore, this study provides an accurate label-free method to detect thrombin in real-time with high affinity and specificity.
Thematic Areas: Zootecnia / recursos pesqueiros Química Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Linguística e literatura Letras / linguística Interdisciplinar Instruments & instrumentation Instrumentation Information systems Geografía Geociências Farmacia Engineering, electrical & electronic Engenharias iv Engenharias iii Engenharias ii Engenharias i Electrochemistry Electrical and electronic engineering Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Biochemistry Atomic and molecular physics, and optics Astronomia / física Arquitetura, urbanismo e design Analytical chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 14248220
Author's mail: laurakaren.acosta@urv.cat josep.ferre@urv.cat lluis.marsal@urv.cat
Author identifier: 0000-0002-5210-5133 0000-0002-5976-1408
Record's date: 2024-10-12
Journal volume: 19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/1424-8220/19/20/4543
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Sensors. 19 (20): 4543-
APA: Pol, Laura; Karen Acosta, Laura; Ferre-Borrull, Josep; Marsal, Lluis F (2019). Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection. Sensors, 19(20), 4543-. DOI: 10.3390/s19204543
Article's DOI: 10.3390/s19204543
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications
Repositori URV