Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection

Autor según el artículo: Pol, Laura; Karen Acosta, Laura; Ferre-Borrull, Josep; Marsal, Lluis F

Departamento: Enginyeria Electrònica, Elèctrica i Automàtica

e-ISSN: 1424-8220

Autor/es de la URV: Acosta Capilla, Laura Karen / Ferré Borrull, José / Marsal Garví, Luis Francisco / POL AIRA, LAURA

Palabras clave: Thrombin aptamer; Thrombin; Streptavidin; Selection; Rifs; Perspective; Nanoporous anodic alumina; Nanopores; Molecules; Limit of detection; Interferometry; Electrodes; Electrochemical detection; Biosensing techniques; Biosensing; Bind; Aptamers, nucleotide; Aptamers; Aluminum oxide; streptavidin; rifs; nanoporous anodic alumina; biosensing; aptamers

Resumen: Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient manner. These chemical modifications must be well defined. In this work we propose nanoporous anodic alumina (NAA) chemically modified with streptavidin as a platform for aptamer immobilization. The immobilization of biotinylated thrombin binding aptamer (TBA) was monitored in real time by means of reflective interferometric spectroscopy (RIfS). The study has permitted to characterize in real time the path to immobilize TBA on the inner pore walls of NAA. Furthermore, this study provides an accurate label-free method to detect thrombin in real-time with high affinity and specificity.

Áreas temáticas: Zootecnia / recursos pesqueiros; Química; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Materiais; Matemática / probabilidade e estatística; Linguística e literatura; Letras / linguística; Interdisciplinar; Instruments & instrumentation; Instrumentation; Information systems; Geografía; Geociências; Farmacia; Engineering, electrical & electronic; Engenharias iv; Engenharias iii; Engenharias ii; Engenharias i; Electrochemistry; Electrical and electronic engineering; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência de alimentos; Ciência da computação; Chemistry, analytical; Biotecnología; Biodiversidade; Biochemistry; Atomic and molecular physics, and optics; Astronomia / física; Arquitetura, urbanismo e design; Analytical chemistry

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14248220

Direcció de correo del autor: laurakaren.acosta@urv.cat; josep.ferre@urv.cat; lluis.marsal@urv.cat

Fecha de alta del registro: 2024-10-12

Volumen de revista: 19

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/1424-8220/19/20/4543

URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Sensors. 19 (20): 4543-

Referencia de l'ítem segons les normes APA: Pol, Laura; Karen Acosta, Laura; Ferre-Borrull, Josep; Marsal, Lluis F (2019). Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection. Sensors, 19(20), 4543-. DOI: 10.3390/s19204543

DOI del artículo: 10.3390/s19204543

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2019

Tipo de publicación: Journal Publications