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Comparison of polysaccharide-based and protein-based chiral liquid chromatography columns for enantioseparation of drugs

  • Dades identificatives

    Identificador: imarina:6157901
    Autors:
    Fu, YandiBorrull, FrancescFontanals, NuriaMarce, Rosa Maria
    Resum:
    © 2020 Wiley Periodicals, Inc. Two different columns—Lux Cellulose-1 and Chiralpak CBH—were evaluated for their chiral recognition abilities for eight drugs comprising three β-blockers, one antacid, and four cathinones in polar-organic elution mode and reversed-phase elution mode, respectively. The factors that affected the enantioseparation were tested and optimized to develop a suitable chiral separation method whose LC conditions are compatible with MS detection. In polar-organic elution mode with the Lux Cellulose-1 column, methanol and acetonitrile were tested as the main components of the mobile phase. In addition, the effects of adding isopropanol as organic modifier, acidic additives (formic acid), and basic additives (diethylamine) were evaluated. In reversed-phase elution mode with the Chiralpak CBH column, the effect of type and concentration of organic modifier (isopropanol, acetonitrile, and methanol), the mobile phase pH (6.4 and 5.0), and buffer concentration (1mM-20mM ammonium acetate) were evaluated. The best enantioseparation was achieved with the Chiralpak CBH column with a mobile phase composed of 5mM ammonium acetate aqueous (pH = 6.4)/methanol (95/5, v/v) at a flow rate of 0.1 mL/min and a temperature of 30°C. Under these conditions, six of eight chiral drugs were baseline separated.
  • Altres:

    Autor segons l'article: Fu, Yandi; Borrull, Francesc; Fontanals, Nuria; Marce, Rosa Maria
    Departament: Química Analítica i Química Orgànica
    Autor/s de la URV: Borrull Ballarín, Francesc / Fontanals Torroja, Núria / Fu, Yandi / Marcé Recasens, Rosa Maria
    Paraules clau: Waste-water Temperature Stereoisomerism Stationary phases Separation Selectors Reversed-phase Polysaccharides Polar-organic mobile phase Phenylcarbamates Pharmaceuticals Pharmaceutical preparations Lux cellulose-1 column Hydrogen-ion concentration Fate Enantioseparation Enantiomeric analysis Chiralpak cbh column Chiral drugs Cellulose Blockers Adrenergic beta-antagonists Acetonitriles Acetonitrile Abuse 2-propanol polar-organic mobile phase lux cellulose-1 column enantioseparation chiralpak cbh column chiral drugs
    Resum: © 2020 Wiley Periodicals, Inc. Two different columns—Lux Cellulose-1 and Chiralpak CBH—were evaluated for their chiral recognition abilities for eight drugs comprising three β-blockers, one antacid, and four cathinones in polar-organic elution mode and reversed-phase elution mode, respectively. The factors that affected the enantioseparation were tested and optimized to develop a suitable chiral separation method whose LC conditions are compatible with MS detection. In polar-organic elution mode with the Lux Cellulose-1 column, methanol and acetonitrile were tested as the main components of the mobile phase. In addition, the effects of adding isopropanol as organic modifier, acidic additives (formic acid), and basic additives (diethylamine) were evaluated. In reversed-phase elution mode with the Chiralpak CBH column, the effect of type and concentration of organic modifier (isopropanol, acetonitrile, and methanol), the mobile phase pH (6.4 and 5.0), and buffer concentration (1mM-20mM ammonium acetate) were evaluated. The best enantioseparation was achieved with the Chiralpak CBH column with a mobile phase composed of 5mM ammonium acetate aqueous (pH = 6.4)/methanol (95/5, v/v) at a flow rate of 0.1 mL/min and a temperature of 30°C. Under these conditions, six of eight chiral drugs were baseline separated.
    Àrees temàtiques: Spectroscopy Saúde coletiva Química Pharmacology & pharmacy Pharmacology Organic chemistry Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Drug discovery Ciências biológicas iii Ciências biológicas ii Ciências ambientais Chemistry, organic Chemistry, medicinal Chemistry, analytical Catalysis Biotecnología Astronomia / física Analytical chemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 08990042
    Adreça de correu electrònic de l'autor: yandi.fu@estudiants.urv.cat nuria.fontanals@urv.cat nuria.fontanals@urv.cat rosamaria.marce@urv.cat francesc.borrull@urv.cat
    Identificador de l'autor: 0000-0002-7534-1964 0000-0002-7534-1964 0000-0002-5667-2899 0000-0003-2718-9336
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
    Enllaç font original: https://onlinelibrary.wiley.com/doi/full/10.1002/chir.23198
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Chirality. 32 (6): 876-884
    Referència de l'ítem segons les normes APA: Fu, Yandi; Borrull, Francesc; Fontanals, Nuria; Marce, Rosa Maria (2020). Comparison of polysaccharide-based and protein-based chiral liquid chromatography columns for enantioseparation of drugs. Chirality, 32(6), 876-884. DOI: 10.1002/chir.23198
    DOI de l'article: 10.1002/chir.23198
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2020
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Analytical Chemistry,Catalysis,Chemistry, Analytical,Chemistry, Medicinal,Chemistry, Organic,Drug Discovery,Organic Chemistry,Pharmacology,Pharmacology & Pharmacy,Spectroscopy
    Waste-water
    Temperature
    Stereoisomerism
    Stationary phases
    Separation
    Selectors
    Reversed-phase
    Polysaccharides
    Polar-organic mobile phase
    Phenylcarbamates
    Pharmaceuticals
    Pharmaceutical preparations
    Lux cellulose-1 column
    Hydrogen-ion concentration
    Fate
    Enantioseparation
    Enantiomeric analysis
    Chiralpak cbh column
    Chiral drugs
    Cellulose
    Blockers
    Adrenergic beta-antagonists
    Acetonitriles
    Acetonitrile
    Abuse
    2-propanol
    polar-organic mobile phase
    lux cellulose-1 column
    enantioseparation
    chiralpak cbh column
    chiral drugs
    Spectroscopy
    Saúde coletiva
    Química
    Pharmacology & pharmacy
    Pharmacology
    Organic chemistry
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    General medicine
    Farmacia
    Engenharias iv
    Drug discovery
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências ambientais
    Chemistry, organic
    Chemistry, medicinal
    Chemistry, analytical
    Catalysis
    Biotecnología
    Astronomia / física
    Analytical chemistry
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