Autor según el artículo: Fu, Yandi; Borrull, Francesc; Fontanals, Nuria; Marce, Rosa Maria
Departamento: Química Analítica i Química Orgànica
Autor/es de la URV: Borrull Ballarín, Francesc / Fontanals Torroja, Núria / Fu, Yandi / Marcé Recasens, Rosa Maria
Palabras clave: Waste-water Temperature Stereoisomerism Stationary phases Separation Selectors Reversed-phase Polysaccharides Polar-organic mobile phase Phenylcarbamates Pharmaceuticals Pharmaceutical preparations Lux cellulose-1 column Hydrogen-ion concentration Fate Enantioseparation Enantiomeric analysis Chiralpak cbh column Chiral drugs Cellulose Blockers Adrenergic beta-antagonists Acetonitriles Acetonitrile Abuse 2-propanol polar-organic mobile phase lux cellulose-1 column enantioseparation chiralpak cbh column chiral drugs
Resumen: © 2020 Wiley Periodicals, Inc. Two different columns—Lux Cellulose-1 and Chiralpak CBH—were evaluated for their chiral recognition abilities for eight drugs comprising three β-blockers, one antacid, and four cathinones in polar-organic elution mode and reversed-phase elution mode, respectively. The factors that affected the enantioseparation were tested and optimized to develop a suitable chiral separation method whose LC conditions are compatible with MS detection. In polar-organic elution mode with the Lux Cellulose-1 column, methanol and acetonitrile were tested as the main components of the mobile phase. In addition, the effects of adding isopropanol as organic modifier, acidic additives (formic acid), and basic additives (diethylamine) were evaluated. In reversed-phase elution mode with the Chiralpak CBH column, the effect of type and concentration of organic modifier (isopropanol, acetonitrile, and methanol), the mobile phase pH (6.4 and 5.0), and buffer concentration (1mM-20mM ammonium acetate) were evaluated. The best enantioseparation was achieved with the Chiralpak CBH column with a mobile phase composed of 5mM ammonium acetate aqueous (pH = 6.4)/methanol (95/5, v/v) at a flow rate of 0.1 mL/min and a temperature of 30°C. Under these conditions, six of eight chiral drugs were baseline separated.
Áreas temáticas: Spectroscopy Saúde coletiva Química Pharmacology & pharmacy Pharmacology Organic chemistry Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Drug discovery Ciências biológicas iii Ciências biológicas ii Ciências ambientais Chemistry, organic Chemistry, medicinal Chemistry, analytical Catalysis Biotecnología Astronomia / física Analytical chemistry
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 08990042
Direcció de correo del autor: yandi.fu@estudiants.urv.cat nuria.fontanals@urv.cat nuria.fontanals@urv.cat rosamaria.marce@urv.cat francesc.borrull@urv.cat
Identificador del autor: 0000-0002-7534-1964 0000-0002-7534-1964 0000-0002-5667-2899 0000-0003-2718-9336
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/full/10.1002/chir.23198
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Chirality. 32 (6): 876-884
Referencia de l'ítem segons les normes APA: Fu, Yandi; Borrull, Francesc; Fontanals, Nuria; Marce, Rosa Maria (2020). Comparison of polysaccharide-based and protein-based chiral liquid chromatography columns for enantioseparation of drugs. Chirality, 32(6), 876-884. DOI: 10.1002/chir.23198
DOI del artículo: 10.1002/chir.23198
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2020
Tipo de publicación: Journal Publications
Repositori URV