Autor según el artículo: Fu Y; Borrull F; Fontanals N; Marcé RM

Departamento: Química Analítica i Química Orgànica

Autor/es de la URV: Borrull Ballarín, Francesc / Fontanals Torroja, Núria / Marcé Recasens, Rosa Maria

Palabras clave: Waste-water Stationary phases Separation Selectors Reversed-phase Polar-organic mobile phase Pharmaceuticals Lux cellulose-1 column Fate Enantioseparation Enantiomeric analysis Chiralpak cbh column Chiral drugs Blockers Abuse polar-organic mobile phase lux cellulose-1 column enantioseparation chiralpak cbh column chiral drugs

Resumen: © 2020 Wiley Periodicals, Inc. Two different columns—Lux Cellulose-1 and Chiralpak CBH—were evaluated for their chiral recognition abilities for eight drugs comprising three β-blockers, one antacid, and four cathinones in polar-organic elution mode and reversed-phase elution mode, respectively. The factors that affected the enantioseparation were tested and optimized to develop a suitable chiral separation method whose LC conditions are compatible with MS detection. In polar-organic elution mode with the Lux Cellulose-1 column, methanol and acetonitrile were tested as the main components of the mobile phase. In addition, the effects of adding isopropanol as organic modifier, acidic additives (formic acid), and basic additives (diethylamine) were evaluated. In reversed-phase elution mode with the Chiralpak CBH column, the effect of type and concentration of organic modifier (isopropanol, acetonitrile, and methanol), the mobile phase pH (6.4 and 5.0), and buffer concentration (1mM-20mM ammonium acetate) were evaluated. The best enantioseparation was achieved with the Chiralpak CBH column with a mobile phase composed of 5mM ammonium acetate aqueous (pH = 6.4)/methanol (95/5, v/v) at a flow rate of 0.1 mL/min and a temperature of 30°C. Under these conditions, six of eight chiral drugs were baseline separated.

Áreas temáticas: Spectroscopy Saúde coletiva Química Pharmacology & pharmacy Pharmacology Organic chemistry Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Drug discovery Ciências biológicas iii Ciências biológicas ii Ciências ambientais Chemistry, organic Chemistry, medicinal Chemistry, analytical Catalysis Biotecnología Astronomia / física Analytical chemistry

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 08990042

Direcció de correo del autor: nuria.fontanals@urv.cat francesc.borrull@urv.cat rosamaria.marce@urv.cat nuria.fontanals@urv.cat

Identificador del autor: 0000-0002-7534-1964 0000-0003-2718-9336 0000-0002-5667-2899 0000-0002-7534-1964

Fecha de alta del registro: 2023-02-22

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/full/10.1002/chir.23198

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Chirality. 32 (6): 876-884

Referencia de l'ítem segons les normes APA: Fu Y; Borrull F; Fontanals N; Marcé RM (2020). Comparison of polysaccharide-based and protein-based chiral liquid chromatography columns for enantioseparation of drugs. Chirality, 32(6), 876-884. DOI: 10.1002/chir.23198

DOI del artículo: 10.1002/chir.23198

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2020

Tipo de publicación: Journal Publications