Articles producció científica> Enginyeria Química

Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor

  • Dades identificatives

    Identificador: imarina:6249287
    Autors:
    Leonardo, SandraGaiani, GretaTsumuraya, TakeshiHirama, MasahiroTurquet, JeanSagrista, NuriaRambla-Alegre, MariaFlores, CintiaCaixach, JosepDiogene, JorgeO'Sullivan, Ciara KAlcaraz, CarlesCampas, Monica
    Resum:
    The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PoIyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 5 1-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 mu g/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.
  • Altres:

    Autor segons l'article: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, Josep; Diogene, Jorge; O'Sullivan, Ciara K; Alcaraz, Carles; Campas, Monica
    Departament: Enginyeria Química
    Autor/s de la URV: CAMPAS HOMS, MONICA / O'SULLIVAN, CIARA KATHLEEN
    Paraules clau: Islands ne atlantic toxins sandwich immunoassay ocean monoclonal-antibodies immunoassay stick test gambierdiscus fish ciguatera 1st report
    Resum: The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PoIyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 5 1-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 mu g/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.
    Àrees temàtiques: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0003-2700
    Adreça de correu electrònic de l'autor: monica.campas@urv.cat
    Identificador de l'autor: 0000-0002-1220-7100
    Pàgina final: 4865
    Data d'alta del registre: 2024-10-26
    Volum de revista: 92
    Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
    Enllaç font original: https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b04499
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Analytical Chemistry. 92 (7): 4858-4865
    Referència de l'ítem segons les normes APA: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, (2020). Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor. Analytical Chemistry, 92(7), 4858-4865. DOI: 10.1021/acs.analchem.9b04499
    DOI de l'article: 10.1021/acs.analchem.9b04499
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2020
    Pàgina inicial: 4858
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Analytical Chemistry,Chemistry, Analytical
    Islands ne atlantic
    toxins
    sandwich immunoassay
    ocean
    monoclonal-antibodies
    immunoassay stick test
    gambierdiscus
    fish
    ciguatera
    1st report
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
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