Autor segons l'article: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, Josep; Diogene, Jorge; O'Sullivan, Ciara K.; Alcaraz, Carles; Campas, Monica;
Departament: Enginyeria Química
Autor/s de la URV: CAMPAS HOMS, MONICA / O'SULLIVAN, CIARA KATHLEEN
Paraules clau: Islands ne atlantic toxins sandwich immunoassay ocean monoclonal-antibodies immunoassay stick test gambierdiscus fish ciguatera 1st report
Resum: The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PoIyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 5 1-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 mu g/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.
Àrees temàtiques: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0003-2700
Adreça de correu electrònic de l'autor: monica.campas@urv.cat
Identificador de l'autor: 0000-0002-1220-7100
Pàgina final: 4865
Data d'alta del registre: 2022-11-19
Volum de revista: 92
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b04499
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Analytical Chemistry. 92 (7): 4858-4865
Referència de l'ítem segons les normes APA: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, (2020). Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor. Analytical Chemistry, 92(7), 4858-4865. DOI: 10.1021/acs.analchem.9b04499
DOI de l'article: 10.1021/acs.analchem.9b04499
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2020
Pàgina inicial: 4858
Tipus de publicació: Journal Publications
Repositori URV