Articles producció científica> Enginyeria Química

Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor

  • Identification data

    Identifier: imarina:6249287
  • Authors:

    Leonardo, Sandra
    Gaiani, Greta
    Tsumuraya, Takeshi
    Hirama, Masahiro
    Turquet, Jean
    Sagrista, Nuria
    Rambla-Alegre, Maria
    Flores, Cintia
    Caixach, Josep
    Diogene, Jorge
    O'Sullivan, Ciara K.
    Alcaraz, Carles
    Campas, Monica
  • Others:

    Author, as appears in the article.: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, Josep; Diogene, Jorge; O'Sullivan, Ciara K.; Alcaraz, Carles; Campas, Monica;
    Department: Enginyeria Química
    URV's Author/s: CAMPAS HOMS, MONICA / O'SULLIVAN, CIARA KATHLEEN
    Keywords: Islands ne atlantic toxins sandwich immunoassay ocean monoclonal-antibodies immunoassay stick test gambierdiscus fish ciguatera 1st report
    Abstract: The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PoIyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 5 1-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 mu g/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.
    Thematic Areas: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0003-2700
    Author's mail: monica.campas@urv.cat
    Author identifier: 0000-0002-1220-7100
    Last page: 4865
    Record's date: 2022-11-19
    Journal volume: 92
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b04499
    Papper original source: Analytical Chemistry. 92 (7): 4858-4865
    APA: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, (2020). Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor. Analytical Chemistry, 92(7), 4858-4865. DOI: 10.1021/acs.analchem.9b04499
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1021/acs.analchem.9b04499
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    First page: 4858
    Publication Type: Journal Publications
  • Keywords:

    Analytical Chemistry,Chemistry, Analytical
    Islands ne atlantic
    toxins
    sandwich immunoassay
    ocean
    monoclonal-antibodies
    immunoassay stick test
    gambierdiscus
    fish
    ciguatera
    1st report
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
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