Autor segons l'article: Evans J; McCarthy J; Torres-Fuentes C; Cryan J; Ogier J; Darcy R; Watson R; O'Driscoll C
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Torres Fuentes, Cristina
Resum: © 2015 Macmillan Publishers Limited All rights reserved. Prostate cancer is the most common cancer in men of the western world. To date, no effective treatment exists for metastatic prostate cancer and consequently, there is an urgent need to develop new and improved therapeutics. In recent years, the therapeutic potential of RNA interference (RNAi) has been extensively explored in a wide range of diseases including prostate cancer using numerous gene delivery vectors. The aims of this study were to investigate the ability of a non-viral modified cyclodextrin (CD) vector to deliver siRNA to the highly metastatic PC-3 prostate cancer cell line, to quantify the resulting knockdown of the two target genes (RelA and SRF) and to study the effects of the silencing on metastasis. Data from a Matrigel in vitro invasion assay indicated that the silencing of the target genes achieved by the CD vector resulted in significant reductions (P=0.0001) in the metastatic potential of these cells. As the silencing of these target genes was shown not to have a negative impact on cell viability, we hypothesise that the mechanism of invasion inhibition is due, in part, to the significant reduction observed (P≤0.0001) in the level of pro-inflammatory cytokine, MMP9, which is known to be implicated in the metastasis of prostate cancer.
Àrees temàtiques: Biochemistry & molecular biology Biotechnology & applied microbiology Ciências biológicas iii General medicine Genetics Genetics & heredity Medicina i Medicina iii Medicine, research & experimental Molecular biology Molecular medicine Odontología Pharmacology & pharmacy
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: cristina.torres@urv.cat
ISSN: 09697128
Identificador de l'autor: 0000-0002-2917-6910
Data d'alta del registre: 2023-02-19
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.nature.com/articles/gt201550
Referència a l'article segons font original: Gene Therapy. 22 (10): 802-810
Referència de l'ítem segons les normes APA: Evans J; McCarthy J; Torres-Fuentes C; Cryan J; Ogier J; Darcy R; Watson R; O'Driscoll C (2015). Cyclodextrin mediated delivery of NF-κB and SRF siRNA reduces the invasion potential of prostate cancer cells in vitro. Gene Therapy, 22(10), 802-810. DOI: 10.1038/gt.2015.50
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1038/gt.2015.50
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2015
Tipus de publicació: Journal Publications
Repositori URV