Articles producció científica> Bioquímica i Biotecnologia

Cyclodextrin mediated delivery of NF-κB and SRF siRNA reduces the invasion potential of prostate cancer cells in vitro

  • Datos identificativos

    Identificador: imarina:6388360
    Autores:
    Evans JMcCarthy JTorres-Fuentes CCryan JOgier JDarcy RWatson RO'Driscoll C
    Resumen:
    © 2015 Macmillan Publishers Limited All rights reserved. Prostate cancer is the most common cancer in men of the western world. To date, no effective treatment exists for metastatic prostate cancer and consequently, there is an urgent need to develop new and improved therapeutics. In recent years, the therapeutic potential of RNA interference (RNAi) has been extensively explored in a wide range of diseases including prostate cancer using numerous gene delivery vectors. The aims of this study were to investigate the ability of a non-viral modified cyclodextrin (CD) vector to deliver siRNA to the highly metastatic PC-3 prostate cancer cell line, to quantify the resulting knockdown of the two target genes (RelA and SRF) and to study the effects of the silencing on metastasis. Data from a Matrigel in vitro invasion assay indicated that the silencing of the target genes achieved by the CD vector resulted in significant reductions (P=0.0001) in the metastatic potential of these cells. As the silencing of these target genes was shown not to have a negative impact on cell viability, we hypothesise that the mechanism of invasion inhibition is due, in part, to the significant reduction observed (P≤0.0001) in the level of pro-inflammatory cytokine, MMP9, which is known to be implicated in the metastasis of prostate cancer.
  • Otros:

    Autor según el artículo: Evans J; McCarthy J; Torres-Fuentes C; Cryan J; Ogier J; Darcy R; Watson R; O'Driscoll C
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: Torres Fuentes, Cristina
    Resumen: © 2015 Macmillan Publishers Limited All rights reserved. Prostate cancer is the most common cancer in men of the western world. To date, no effective treatment exists for metastatic prostate cancer and consequently, there is an urgent need to develop new and improved therapeutics. In recent years, the therapeutic potential of RNA interference (RNAi) has been extensively explored in a wide range of diseases including prostate cancer using numerous gene delivery vectors. The aims of this study were to investigate the ability of a non-viral modified cyclodextrin (CD) vector to deliver siRNA to the highly metastatic PC-3 prostate cancer cell line, to quantify the resulting knockdown of the two target genes (RelA and SRF) and to study the effects of the silencing on metastasis. Data from a Matrigel in vitro invasion assay indicated that the silencing of the target genes achieved by the CD vector resulted in significant reductions (P=0.0001) in the metastatic potential of these cells. As the silencing of these target genes was shown not to have a negative impact on cell viability, we hypothesise that the mechanism of invasion inhibition is due, in part, to the significant reduction observed (P≤0.0001) in the level of pro-inflammatory cytokine, MMP9, which is known to be implicated in the metastasis of prostate cancer.
    Áreas temáticas: Pharmacology & pharmacy Odontología Molecular medicine Molecular biology Medicine, research & experimental Medicina iii Medicina i Genetics & heredity Genetics General medicine Ciências biológicas iii Biotechnology & applied microbiology Biochemistry & molecular biology
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 09697128
    Direcció de correo del autor: cristina.torres@urv.cat
    Identificador del autor: 0000-0002-2917-6910
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://www.nature.com/articles/gt201550
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Gene Therapy. 22 (10): 802-810
    Referencia de l'ítem segons les normes APA: Evans J; McCarthy J; Torres-Fuentes C; Cryan J; Ogier J; Darcy R; Watson R; O'Driscoll C (2015). Cyclodextrin mediated delivery of NF-κB and SRF siRNA reduces the invasion potential of prostate cancer cells in vitro. Gene Therapy, 22(10), 802-810. DOI: 10.1038/gt.2015.50
    DOI del artículo: 10.1038/gt.2015.50
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2015
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry & Molecular Biology,Biotechnology & Applied Microbiology,Genetics,Genetics & Heredity,Medicine, Research & Experimental,Molecular Biology,Molecular Medicine,Pharmacology & Pharmacy
    Pharmacology & pharmacy
    Odontología
    Molecular medicine
    Molecular biology
    Medicine, research & experimental
    Medicina iii
    Medicina i
    Genetics & heredity
    Genetics
    General medicine
    Ciências biológicas iii
    Biotechnology & applied microbiology
    Biochemistry & molecular biology
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