Author, as appears in the article.: Evans J; McCarthy J; Torres-Fuentes C; Cryan J; Ogier J; Darcy R; Watson R; O'Driscoll C
Department: Bioquímica i Biotecnologia
URV's Author/s: Torres Fuentes, Cristina
Abstract: © 2015 Macmillan Publishers Limited All rights reserved. Prostate cancer is the most common cancer in men of the western world. To date, no effective treatment exists for metastatic prostate cancer and consequently, there is an urgent need to develop new and improved therapeutics. In recent years, the therapeutic potential of RNA interference (RNAi) has been extensively explored in a wide range of diseases including prostate cancer using numerous gene delivery vectors. The aims of this study were to investigate the ability of a non-viral modified cyclodextrin (CD) vector to deliver siRNA to the highly metastatic PC-3 prostate cancer cell line, to quantify the resulting knockdown of the two target genes (RelA and SRF) and to study the effects of the silencing on metastasis. Data from a Matrigel in vitro invasion assay indicated that the silencing of the target genes achieved by the CD vector resulted in significant reductions (P=0.0001) in the metastatic potential of these cells. As the silencing of these target genes was shown not to have a negative impact on cell viability, we hypothesise that the mechanism of invasion inhibition is due, in part, to the significant reduction observed (P≤0.0001) in the level of pro-inflammatory cytokine, MMP9, which is known to be implicated in the metastasis of prostate cancer.
Thematic Areas: Pharmacology & pharmacy Odontología Molecular medicine Molecular biology Medicine, research & experimental Medicina iii Medicina i Genetics & heredity Genetics General medicine Ciências biológicas iii Biotechnology & applied microbiology Biochemistry & molecular biology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 09697128
Author's mail: cristina.torres@urv.cat
Author identifier: 0000-0002-2917-6910
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.nature.com/articles/gt201550
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Gene Therapy. 22 (10): 802-810
APA: Evans J; McCarthy J; Torres-Fuentes C; Cryan J; Ogier J; Darcy R; Watson R; O'Driscoll C (2015). Cyclodextrin mediated delivery of NF-κB and SRF siRNA reduces the invasion potential of prostate cancer cells in vitro. Gene Therapy, 22(10), 802-810. DOI: 10.1038/gt.2015.50
Article's DOI: 10.1038/gt.2015.50
Entity: Universitat Rovira i Virgili
Journal publication year: 2015
Publication Type: Journal Publications
Repositori URV