Articles producció científica> Medicina i Cirurgia

Proteomic Profile of Unstable Atheroma Plaque: Increased Neutrophil Defensin 1, Clusterin, and Apolipoprotein e Levels in Carotid Secretome

  • Dades identificatives

    Identificador: imarina:6388593
    Autors:
    Aragones, GemmaAuguet, TeresaGuiu-Jurado, EstherBerlanga, AlbaCurriu, MartaMartinez, SalomeAlibalic, AjlaAguilar, CarmenHernandez, EstebanCamara, Maria-LuisaCanela, NuriaHerrero, PolRuyra, XavierMartin-Paredero, VicenteRichart, Cristobal
    Resum:
    © 2016 American Chemical Society. Because of the clinical significance of carotid atherosclerosis, the search for novel biomarkers has become a priority. The aim of the present study was to compare the protein secretion profile of the carotid atherosclerotic plaque (CAP, n = 12) and nonatherosclerotic mammary artery (MA, n = 10) secretomes. We used a nontargeted proteomic approach that incorporated tandem immunoaffinity depletion, iTRAQ labeling, and nanoflow liquid chromatography coupled to high-resolution mass spectrometry. In total, 162 proteins were quantified, of which 25 showed statistically significant differences in secretome levels between carotid atherosclerotic plaque and nondiseased mammary artery. We found increased levels of neutrophil defensin 1, apolipoprotein E, clusterin, and zinc-alpha-2-glycoprotein in CAP secretomes. Results were validated by ELISA assays. Also, differentially secreted proteins are involved in pathways such as focal adhesion and leukocyte transendothelial migration. In conclusion, this study provides a subset of identified proteins that are differently expressed in secretomes of clinical significance.
  • Altres:

    Autor segons l'article: Aragones, Gemma; Auguet, Teresa; Guiu-Jurado, Esther; Berlanga, Alba; Curriu, Marta; Martinez, Salome; Alibalic, Ajla; Aguilar, Carmen; Hernandez, Esteban; Camara, Maria-Luisa; Canela, Nuria; Herrero, Pol; Ruyra, Xavier; Martin-Paredero, Vicente; Richart, Cristobal
    Departament: Ciències Mèdiques Bàsiques Medicina i Cirurgia
    Autor/s de la URV: Aguilar Crespillo, Carmen Isabel / AGUILAR MARMOL, CARME / ARAGONÈS BARGALLÓ, GEMMA / Aragonès Bargalló, Gerard / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / GUIU JURADO, ESTHER / HERRERO GIL, POL / Martín Paredero, Vicente / Martínez González, María Salomé / Richart Jurado, Cristobal Manuel
    Paraules clau: Secretome Proteomic Atherosclerosis Atheroma plaque proteomic atherosclerosis atheroma plaque
    Resum: © 2016 American Chemical Society. Because of the clinical significance of carotid atherosclerosis, the search for novel biomarkers has become a priority. The aim of the present study was to compare the protein secretion profile of the carotid atherosclerotic plaque (CAP, n = 12) and nonatherosclerotic mammary artery (MA, n = 10) secretomes. We used a nontargeted proteomic approach that incorporated tandem immunoaffinity depletion, iTRAQ labeling, and nanoflow liquid chromatography coupled to high-resolution mass spectrometry. In total, 162 proteins were quantified, of which 25 showed statistically significant differences in secretome levels between carotid atherosclerotic plaque and nondiseased mammary artery. We found increased levels of neutrophil defensin 1, apolipoprotein E, clusterin, and zinc-alpha-2-glycoprotein in CAP secretomes. Results were validated by ELISA assays. Also, differentially secreted proteins are involved in pathways such as focal adhesion and leukocyte transendothelial migration. In conclusion, this study provides a subset of identified proteins that are differently expressed in secretomes of clinical significance.
    Àrees temàtiques: Química Odontología Medicina veterinaria Medicina ii Medicina i Interdisciplinar General chemistry Farmacia Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Biochemistry Biochemical research methods Astronomia / física
    ISSN: 15353893
    Adreça de correu electrònic de l'autor: mariasalome.martinez@urv.cat gerard.aragones@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
    Identificador de l'autor: 0000-0001-6185-2889 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
    Data d'alta del registre: 2024-09-28
    Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
    Enllaç font original: https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.5b00936
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Journal Of Proteome Research. 15 (3): 933-944
    Referència de l'ítem segons les normes APA: Aragones, Gemma; Auguet, Teresa; Guiu-Jurado, Esther; Berlanga, Alba; Curriu, Marta; Martinez, Salome; Alibalic, Ajla; Aguilar, Carmen; Hernandez, Est (2016). Proteomic Profile of Unstable Atheroma Plaque: Increased Neutrophil Defensin 1, Clusterin, and Apolipoprotein e Levels in Carotid Secretome. Journal Of Proteome Research, 15(3), 933-944. DOI: 10.1021/acs.jproteome.5b00936
    DOI de l'article: 10.1021/acs.jproteome.5b00936
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2016
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemical Research Methods,Biochemistry,Chemistry (Miscellaneous)
    Secretome
    Proteomic
    Atherosclerosis
    Atheroma plaque
    proteomic
    atherosclerosis
    atheroma plaque
    Química
    Odontología
    Medicina veterinaria
    Medicina ii
    Medicina i
    Interdisciplinar
    General chemistry
    Farmacia
    Engenharias iii
    Engenharias ii
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry (miscellaneous)
    Chemistry (all)
    Biotecnología
    Biodiversidade
    Biochemistry
    Biochemical research methods
    Astronomia / física
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