Author, as appears in the article.: Aragones, Gemma; Auguet, Teresa; Guiu-Jurado, Esther; Berlanga, Alba; Curriu, Marta; Martinez, Salome; Alibalic, Ajla; Aguilar, Carmen; Hernandez, Esteban; Camara, Maria-Luisa; Canela, Nuria; Herrero, Pol; Ruyra, Xavier; Martin-Paredero, Vicente; Richart, Cristobal
Department: Ciències Mèdiques Bàsiques Medicina i Cirurgia
URV's Author/s: Aguilar Crespillo, Carmen Isabel / AGUILAR MARMOL, CARME / ARAGONÈS BARGALLÓ, GEMMA / Aragonès Bargalló, Gerard / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / GUIU JURADO, ESTHER / HERRERO GIL, POL / Martín Paredero, Vicente / Martínez González, María Salomé / Richart Jurado, Cristobal Manuel
Keywords: Secretome Proteomic Atherosclerosis Atheroma plaque proteomic atherosclerosis atheroma plaque
Abstract: © 2016 American Chemical Society. Because of the clinical significance of carotid atherosclerosis, the search for novel biomarkers has become a priority. The aim of the present study was to compare the protein secretion profile of the carotid atherosclerotic plaque (CAP, n = 12) and nonatherosclerotic mammary artery (MA, n = 10) secretomes. We used a nontargeted proteomic approach that incorporated tandem immunoaffinity depletion, iTRAQ labeling, and nanoflow liquid chromatography coupled to high-resolution mass spectrometry. In total, 162 proteins were quantified, of which 25 showed statistically significant differences in secretome levels between carotid atherosclerotic plaque and nondiseased mammary artery. We found increased levels of neutrophil defensin 1, apolipoprotein E, clusterin, and zinc-alpha-2-glycoprotein in CAP secretomes. Results were validated by ELISA assays. Also, differentially secreted proteins are involved in pathways such as focal adhesion and leukocyte transendothelial migration. In conclusion, this study provides a subset of identified proteins that are differently expressed in secretomes of clinical significance.
Thematic Areas: Química Odontología Medicina veterinaria Medicina ii Medicina i Interdisciplinar General chemistry Farmacia Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Biochemistry Biochemical research methods Astronomia / física
ISSN: 15353893
Author's mail: mariasalome.martinez@urv.cat gerard.aragones@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
Author identifier: 0000-0001-6185-2889 0000-0002-4440-562X 0000-0002-4440-562X https://orcid.org/0000-0003-0396-6428 0000-0003-0396-6428
Record's date: 2024-12-21
Papper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of Proteome Research. 15 (3): 933-944
APA: Aragones, Gemma; Auguet, Teresa; Guiu-Jurado, Esther; Berlanga, Alba; Curriu, Marta; Martinez, Salome; Alibalic, Ajla; Aguilar, Carmen; Hernandez, Est (2016). Proteomic Profile of Unstable Atheroma Plaque: Increased Neutrophil Defensin 1, Clusterin, and Apolipoprotein e Levels in Carotid Secretome. Journal Of Proteome Research, 15(3), 933-944. DOI: 10.1021/acs.jproteome.5b00936
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV