Autor según el artículo: Aragonès G; Auguet T; Guiu-Jurado E; Berlanga A; Curriu M; Martinez S; Alibalic A; Aguilar C; Hernández E; Camara M; Canela N; Herrero P; Ruyra X; Martín-Paredero V; Richart C

Departamento: Ciències Mèdiques Bàsiques Medicina i Cirurgia

Autor/es de la URV: Aguilar Crespillo, Carmen Isabel / ARAGONÈS BARGALLÓ, GEMMA / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / GUIU JURADO, ESTHER / HERRERO GIL, POL / Martín Paredero, Vicente / Martínez González, María Salomé / Richart Jurado, Cristobal Manuel

Palabras clave: Secretome Proteomic Atherosclerosis Atheroma plaque proteomic atherosclerosis atheroma plaque

Resumen: © 2016 American Chemical Society. Because of the clinical significance of carotid atherosclerosis, the search for novel biomarkers has become a priority. The aim of the present study was to compare the protein secretion profile of the carotid atherosclerotic plaque (CAP, n = 12) and nonatherosclerotic mammary artery (MA, n = 10) secretomes. We used a nontargeted proteomic approach that incorporated tandem immunoaffinity depletion, iTRAQ labeling, and nanoflow liquid chromatography coupled to high-resolution mass spectrometry. In total, 162 proteins were quantified, of which 25 showed statistically significant differences in secretome levels between carotid atherosclerotic plaque and nondiseased mammary artery. We found increased levels of neutrophil defensin 1, apolipoprotein E, clusterin, and zinc-alpha-2-glycoprotein in CAP secretomes. Results were validated by ELISA assays. Also, differentially secreted proteins are involved in pathways such as focal adhesion and leukocyte transendothelial migration. In conclusion, this study provides a subset of identified proteins that are differently expressed in secretomes of clinical significance.

Áreas temáticas: Química Odontología Medicina veterinaria Medicina ii Medicina i Interdisciplinar General chemistry Farmacia Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Biochemistry Biochemical research methods Astronomia / física

ISSN: 15353893

Direcció de correo del autor: vicente.martin@urv.cat mariasalome.martinez@urv.cat cristobalmanuel.richart@urv.cat mariateresa.auguet@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat

Identificador del autor: 0000-0002-2740-5847 0000-0001-6185-2889 0000-0003-0852-6739 0000-0003-0396-6428 0000-0002-4440-562X 0000-0002-4440-562X

Fecha de alta del registro: 2023-03-11

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.5b00936

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Journal Of Proteome Research. 15 (3): 933-944

Referencia de l'ítem segons les normes APA: Aragonès G; Auguet T; Guiu-Jurado E; Berlanga A; Curriu M; Martinez S; Alibalic A; Aguilar C; Hernández E; Camara M; Canela N; Herrero P; Ruyra X; Mar (2016). Proteomic Profile of Unstable Atheroma Plaque: Increased Neutrophil Defensin 1, Clusterin, and Apolipoprotein e Levels in Carotid Secretome. Journal Of Proteome Research, 15(3), 933-944. DOI: 10.1021/acs.jproteome.5b00936

DOI del artículo: 10.1021/acs.jproteome.5b00936

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Tipo de publicación: Journal Publications