Codi de projecte: CTQ2016-77128-R
Paraules clau: Whole blood detection Potentiometry Paper-based sensor Monitoring glucose Mixed potential Glucose Electrode Electrochemical biosensors potentiometry paper-based sensor mixed potential glucose
Data d'alta del registre: 2023-02-19
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Biosensors & Bioelectronics. 163 (112302): 112302-
Referència de l'ítem segons les normes APA: Cánovas R; Blondeau P; Andrade FJ (2020). Modulating the mixed potential for developing biosensors: Direct potentiometric determination of glucose in whole, undiluted blood. Biosensors & Bioelectronics, 163(112302), 112302-. DOI: 10.1016/j.bios.2020.112302
Acrònim: HOMESENS
Tipus de publicació: Journal Publications
Autor segons l'article: Cánovas R; Blondeau P; Andrade FJ
Departament: Química Analítica i Química Orgànica
Autor/s de la URV: Blondeau, Pascal Jean Claude Leon
Resum: © 2020 Elsevier B.V. The growing demand for tools to generate chemical information in decentralized settings is creating a vast range of opportunities for potentiometric sensors, since their combination of robustness, simplicity of operation and cost can hardly be rivalled by any other technique. In previous works, we have shown that the mixed potential of a Pt electrode can be controlled with analytical purposes using a coating of Nafion, thus providing a way to develop a potentiometric biosensor for glucose. Unfortunately, the linear range of this device did not match the relevant clinical range for glucose in blood. This work presents a novel strategy to control the mixed potential that allows the development of a potentiometric biosensor for the direct detection of glucose in whole, undiluted blood without any sample pretreatment. By changing the ionomer, the analytical response can be tuned, shifting the linear range while keeping the sensitivity. Aquivion, a polyelectrolyte from the same family as Nafion, is used to stabilize the mixed potential of a platinized paper-based electrode, to entrap the enzyme and to reduce the interference from negatively charged species. Factors affecting the generation of the signal and the principle of detection are discussed. Optimization of the biosensor composition was achieved with particular focus on the characterization of the linear range and sensitivity. The accurate measurement of blood sugar levels in a single drop of whole blood with excellent recovery is presented.
Àrees temàtiques: Química Nanoscience and nanotechnology Nanoscience & nanotechnology Medicine (miscellaneous) Medicina ii Medicina i Materiais Interdisciplinar Farmacia Engenharias iv Engenharias iii Engenharias ii Electrochemistry Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Chemistry, analytical Biotecnología Biotechnology & applied microbiology Biotechnology Biophysics Biomedical engineering Biodiversidade Astronomia / física
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0956-5663
Adreça de correu electrònic de l'autor: pascal.blondeau@urv.cat
Identificador de l'autor: 0000-0003-1331-5055
Volum de revista: 163
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0956566320302979?via%3Dihub
Programa de finançament: Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad (Agencia Estatal de Investigación - Ministerio de Economía, Industria y Competitividad)
DOI de l'article: 10.1016/j.bios.2020.112302
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2020
Acció del programa de finançament: Proyecto de I+D
Repositori URV