Articles producció científica> Química Analítica i Química Orgànica

Modulating the mixed potential for developing biosensors: Direct potentiometric determination of glucose in whole, undiluted blood

  • Datos identificativos

    Identificador: imarina:6390143
    Autores:
    Cánovas RBlondeau PAndrade FJ
    Resumen:
    © 2020 Elsevier B.V. The growing demand for tools to generate chemical information in decentralized settings is creating a vast range of opportunities for potentiometric sensors, since their combination of robustness, simplicity of operation and cost can hardly be rivalled by any other technique. In previous works, we have shown that the mixed potential of a Pt electrode can be controlled with analytical purposes using a coating of Nafion, thus providing a way to develop a potentiometric biosensor for glucose. Unfortunately, the linear range of this device did not match the relevant clinical range for glucose in blood. This work presents a novel strategy to control the mixed potential that allows the development of a potentiometric biosensor for the direct detection of glucose in whole, undiluted blood without any sample pretreatment. By changing the ionomer, the analytical response can be tuned, shifting the linear range while keeping the sensitivity. Aquivion, a polyelectrolyte from the same family as Nafion, is used to stabilize the mixed potential of a platinized paper-based electrode, to entrap the enzyme and to reduce the interference from negatively charged species. Factors affecting the generation of the signal and the principle of detection are discussed. Optimization of the biosensor composition was achieved with particular focus on the characterization of the linear range and sensitivity. The accurate measurement of blood sugar levels in a single drop of whole blood with excellent recovery is presented.
  • Otros:

    Código de proyecto: CTQ2016-77128-R
    Palabras clave: Whole blood detection Potentiometry Paper-based sensor Monitoring glucose Mixed potential Glucose Electrode Electrochemical biosensors potentiometry paper-based sensor mixed potential glucose
    Fecha de alta del registro: 2023-02-19
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Referencia al articulo segun fuente origial: Biosensors & Bioelectronics. 163 (112302): 112302-
    Referencia de l'ítem segons les normes APA: Cánovas R; Blondeau P; Andrade FJ (2020). Modulating the mixed potential for developing biosensors: Direct potentiometric determination of glucose in whole, undiluted blood. Biosensors & Bioelectronics, 163(112302), 112302-. DOI: 10.1016/j.bios.2020.112302
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Acrónimo: HOMESENS
    Tipo de publicación: Journal Publications
    Autor según el artículo: Cánovas R; Blondeau P; Andrade FJ
    Departamento: Química Analítica i Química Orgànica
    Autor/es de la URV: Blondeau, Pascal Jean Claude Leon
    Resumen: © 2020 Elsevier B.V. The growing demand for tools to generate chemical information in decentralized settings is creating a vast range of opportunities for potentiometric sensors, since their combination of robustness, simplicity of operation and cost can hardly be rivalled by any other technique. In previous works, we have shown that the mixed potential of a Pt electrode can be controlled with analytical purposes using a coating of Nafion, thus providing a way to develop a potentiometric biosensor for glucose. Unfortunately, the linear range of this device did not match the relevant clinical range for glucose in blood. This work presents a novel strategy to control the mixed potential that allows the development of a potentiometric biosensor for the direct detection of glucose in whole, undiluted blood without any sample pretreatment. By changing the ionomer, the analytical response can be tuned, shifting the linear range while keeping the sensitivity. Aquivion, a polyelectrolyte from the same family as Nafion, is used to stabilize the mixed potential of a platinized paper-based electrode, to entrap the enzyme and to reduce the interference from negatively charged species. Factors affecting the generation of the signal and the principle of detection are discussed. Optimization of the biosensor composition was achieved with particular focus on the characterization of the linear range and sensitivity. The accurate measurement of blood sugar levels in a single drop of whole blood with excellent recovery is presented.
    Áreas temáticas: Química Nanoscience and nanotechnology Nanoscience & nanotechnology Medicine (miscellaneous) Medicina ii Medicina i Materiais Interdisciplinar Farmacia Engenharias iv Engenharias iii Engenharias ii Electrochemistry Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Chemistry, analytical Biotecnología Biotechnology & applied microbiology Biotechnology Biophysics Biomedical engineering Biodiversidade Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0956-5663
    Direcció de correo del autor: pascal.blondeau@urv.cat
    Identificador del autor: 0000-0003-1331-5055
    Volumen de revista: 163
    Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S0956566320302979?via%3Dihub
    Programa de financiación: Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad (Agencia Estatal de Investigación - Ministerio de Economía, Industria y Competitividad)
    DOI del artículo: 10.1016/j.bios.2020.112302
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Acción del progama de financiación: Proyecto de I+D
  • Palabras clave:

    Biomedical Engineering,Biophysics,Biotechnology,Biotechnology & Applied Microbiology,Chemistry, Analytical,Electrochemistry,Medicine (Miscellaneous),Nanoscience & Nanotechnology,Nanoscience and Nanotechnology
    Whole blood detection
    Potentiometry
    Paper-based sensor
    Monitoring glucose
    Mixed potential
    Glucose
    Electrode
    Electrochemical biosensors
    potentiometry
    paper-based sensor
    mixed potential
    glucose
    Química
    Nanoscience and nanotechnology
    Nanoscience & nanotechnology
    Medicine (miscellaneous)
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Electrochemistry
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Chemistry, analytical
    Biotecnología
    Biotechnology & applied microbiology
    Biotechnology
    Biophysics
    Biomedical engineering
    Biodiversidade
    Astronomia / física
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