Código de proyecto: CTQ2016-77128-R
Palabras clave: Whole blood detection Potentiometry Paper-based sensor Monitoring glucose Mixed potential Glucose Electrode Electrochemical biosensors potentiometry paper-based sensor mixed potential glucose
Fecha de alta del registro: 2023-02-19
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Referencia al articulo segun fuente origial: Biosensors & Bioelectronics. 163 (112302): 112302-
Referencia de l'ítem segons les normes APA: Cánovas R; Blondeau P; Andrade FJ (2020). Modulating the mixed potential for developing biosensors: Direct potentiometric determination of glucose in whole, undiluted blood. Biosensors & Bioelectronics, 163(112302), 112302-. DOI: 10.1016/j.bios.2020.112302
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Acrónimo: HOMESENS
Tipo de publicación: Journal Publications
Autor según el artículo: Cánovas R; Blondeau P; Andrade FJ
Departamento: Química Analítica i Química Orgànica
Autor/es de la URV: Blondeau, Pascal Jean Claude Leon
Resumen: © 2020 Elsevier B.V. The growing demand for tools to generate chemical information in decentralized settings is creating a vast range of opportunities for potentiometric sensors, since their combination of robustness, simplicity of operation and cost can hardly be rivalled by any other technique. In previous works, we have shown that the mixed potential of a Pt electrode can be controlled with analytical purposes using a coating of Nafion, thus providing a way to develop a potentiometric biosensor for glucose. Unfortunately, the linear range of this device did not match the relevant clinical range for glucose in blood. This work presents a novel strategy to control the mixed potential that allows the development of a potentiometric biosensor for the direct detection of glucose in whole, undiluted blood without any sample pretreatment. By changing the ionomer, the analytical response can be tuned, shifting the linear range while keeping the sensitivity. Aquivion, a polyelectrolyte from the same family as Nafion, is used to stabilize the mixed potential of a platinized paper-based electrode, to entrap the enzyme and to reduce the interference from negatively charged species. Factors affecting the generation of the signal and the principle of detection are discussed. Optimization of the biosensor composition was achieved with particular focus on the characterization of the linear range and sensitivity. The accurate measurement of blood sugar levels in a single drop of whole blood with excellent recovery is presented.
Áreas temáticas: Química Nanoscience and nanotechnology Nanoscience & nanotechnology Medicine (miscellaneous) Medicina ii Medicina i Materiais Interdisciplinar Farmacia Engenharias iv Engenharias iii Engenharias ii Electrochemistry Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Chemistry, analytical Biotecnología Biotechnology & applied microbiology Biotechnology Biophysics Biomedical engineering Biodiversidade Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0956-5663
Direcció de correo del autor: pascal.blondeau@urv.cat
Identificador del autor: 0000-0003-1331-5055
Volumen de revista: 163
Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S0956566320302979?via%3Dihub
Programa de financiación: Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad (Agencia Estatal de Investigación - Ministerio de Economía, Industria y Competitividad)
DOI del artículo: 10.1016/j.bios.2020.112302
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2020
Acción del progama de financiación: Proyecto de I+D