Autor segons l'article: Satta, G; Atzeni, A; McHugh, T D
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Atzeni, Alessandro
Paraules clau: Whole genome sequencing; Web browser; Tuberculosis; Software; Sequence analysis, dna; Rapid susceptibility testing; Phylogeny; Phylogenetic analysis; Outbreak; Online tools; Mycobacterium tuberculosis; Microbial sensitivity tests; Humans; Genome, bacterial; Diagnosis; Cultures; Computational biology; Bacterial typing techniques; Antitubercular agents; Antibiotic-resistance; rapid susceptibility testing; phylogenetic analysis; online tools; mycobacterium tuberculosis
Resum: Whole genome sequencing (WGS) has the potential to revolutionize the diagnosis of Mycobacterium tuberculosis infection but the lack of bioinformatic expertise among clinical microbiologists is a barrier for adoption. Software products for analysis should be simple, free of charge, able to accept data directly from the sequencer (FASTQ files) and to provide the basic functionalities all-in-one. The main aim of this narrative review is to provide a practical guide for the clinical microbiologist, with little or no practical experience of WGS analysis, with a specific focus on software products tailor-made for M. tuberculosis analysis. With sequencing performed by an external provider, it is now feasible to implement WGS analysis in the routine clinical practice of any microbiology laboratory, with the potential to detect resistance weeks before traditional phenotypic culture methods, but the clinical microbiologist should be aware of the limitations of this approach. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Àrees temàtiques: Saúde coletiva; Química; Odontología; Microbiology (medical); Microbiology; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; Infectious diseases; Farmacia; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Biotecnología; Astronomia / física
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1198743X
Adreça de correu electrònic de l'autor: alessandro.atzeni@urv.cat; alessandro.atzeni@urv.cat
Data d'alta del registre: 2025-02-08
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(16)30392-5/fulltext
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Clinical Microbiology And Infection. 23 (2): 69-72
Referència de l'ítem segons les normes APA: Satta, G; Atzeni, A; McHugh, T D (2017). Mycobacterium tuberculosis and whole genome sequencing: a practical guide and online tools available for the clinical microbiologist. Clinical Microbiology And Infection, 23(2), 69-72. DOI: 10.1016/j.cmi.2016.09.005
DOI de l'article: 10.1016/j.cmi.2016.09.005
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2017
Tipus de publicació: Journal Publications
Repositori URV