Autor según el artículo: Satta, G; Atzeni, A; McHugh, T D
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Atzeni, Alessandro
Palabras clave: Whole genome sequencing; Web browser; Tuberculosis; Software; Sequence analysis, dna; Rapid susceptibility testing; Phylogeny; Phylogenetic analysis; Outbreak; Online tools; Mycobacterium tuberculosis; Microbial sensitivity tests; Humans; Genome, bacterial; Diagnosis; Cultures; Computational biology; Bacterial typing techniques; Antitubercular agents; Antibiotic-resistance; rapid susceptibility testing; phylogenetic analysis; online tools; mycobacterium tuberculosis
Resumen: Whole genome sequencing (WGS) has the potential to revolutionize the diagnosis of Mycobacterium tuberculosis infection but the lack of bioinformatic expertise among clinical microbiologists is a barrier for adoption. Software products for analysis should be simple, free of charge, able to accept data directly from the sequencer (FASTQ files) and to provide the basic functionalities all-in-one. The main aim of this narrative review is to provide a practical guide for the clinical microbiologist, with little or no practical experience of WGS analysis, with a specific focus on software products tailor-made for M. tuberculosis analysis. With sequencing performed by an external provider, it is now feasible to implement WGS analysis in the routine clinical practice of any microbiology laboratory, with the potential to detect resistance weeks before traditional phenotypic culture methods, but the clinical microbiologist should be aware of the limitations of this approach. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Áreas temáticas: Saúde coletiva; Química; Odontología; Microbiology (medical); Microbiology; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; Infectious diseases; Farmacia; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Biotecnología; Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1198743X
Direcció de correo del autor: alessandro.atzeni@urv.cat; alessandro.atzeni@urv.cat
Fecha de alta del registro: 2025-02-08
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(16)30392-5/fulltext
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Clinical Microbiology And Infection. 23 (2): 69-72
Referencia de l'ítem segons les normes APA: Satta, G; Atzeni, A; McHugh, T D (2017). Mycobacterium tuberculosis and whole genome sequencing: a practical guide and online tools available for the clinical microbiologist. Clinical Microbiology And Infection, 23(2), 69-72. DOI: 10.1016/j.cmi.2016.09.005
DOI del artículo: 10.1016/j.cmi.2016.09.005
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2017
Tipo de publicación: Journal Publications
Repositori URV