Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment

Autor segons l'article: Rahman, Faiz Ur; Andree, Karl B; Salas-Masso, Nuria; Fernandez-Tejedor, Margarita; Sanjuan, Anna; Figueras, Maria J; Furones, M Dolors

Departament: Ciències Mèdiques Bàsiques

Autor/s de la URV: Figueras Salvat, María Josefa / Ur Rahman, Faiz / UR, FAIZ

Paraules clau: Water; Taxonomy; Spp.; Sp-nov.; Shellfish; Seawater; Prevalence; Marine biology; Genetic diversity; Food microbiology; Cryaerophilus; Campylobacter; Butzleri; Bacteria; Arcobacter; Animals; Analysis of variance

Resum: Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter-like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter-like spp. from marine and shellfish matrices. For this purpose, 14 different Arcobacter-like spp. mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD420nm) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences (p?<?0.05) among broths were calculated by ANOVA. The results indicated that shellfish-associated Arcobacter-like species growth was significantly higher in Arcobacter broth +?50% ASW and the same media supplemented with lyophilized oysters. This is the first study to have used fresh or lyophilized oyster flesh in the enrichment broth for isolation of shellfish-associated Arcobacter-like spp.

Àrees temàtiques: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Psicología; Odontología; Nutrição; Multidisciplinary sciences; Multidisciplinary; Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Materiais; Matemática / probabilidade e estatística; Letras / linguística; Interdisciplinar; Geografía; Geociências; Farmacia; Engenharias iv; Engenharias iii; Engenharias ii; Enfermagem; Educação física; Educação; Economia; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência de alimentos; Ciência da computação; Biotecnología; Biodiversidade; Astronomia / física

Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 20452322

Adreça de correu electrònic de l'autor: faiz.urrahman@estudiants.urv.cat; mariajose.figueras@urv.cat

Data d'alta del registre: 2025-02-18

Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion

Enllaç font original: https://www.nature.com/articles/s41598-020-71442-8

URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/

Referència a l'article segons font original: Scientific Reports. 10 (1): 14547-

Referència de l'ítem segons les normes APA: Rahman, Faiz Ur; Andree, Karl B; Salas-Masso, Nuria; Fernandez-Tejedor, Margarita; Sanjuan, Anna; Figueras, Maria J; Furones, M Dolors (2020). Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment. Scientific Reports, 10(1), 14547-. DOI: 10.1038/s41598-020-71442-8

DOI de l'article: 10.1038/s41598-020-71442-8

Entitat: Universitat Rovira i Virgili

Any de publicació de la revista: 2020

Tipus de publicació: Journal Publications