Articles producció científica> Ciències Mèdiques Bàsiques

Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment

  • Dades identificatives

    Identificador: imarina:8087228
    Autors:
    Faiz Ur Rahman, Karl B. Andree, Nuria Salas-Massó, Margarita Fernandez-Tejedor, Anna Sanjuan, Maria J. Figueras & M. Dolors Furones
    Resum:
    Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter-like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter-like spp. from marine and shellfish matrices. For this purpose, 14 different Arcobacter-like spp. mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD420nm) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences (p?
  • Altres:

    Autor segons l'article: Faiz Ur Rahman, Karl B. Andree, Nuria Salas-Massó, Margarita Fernandez-Tejedor, Anna Sanjuan, Maria J. Figueras & M. Dolors Furones
    Departament: Ciències Mèdiques Bàsiques
    Autor/s de la URV: Figueras Salvat, María Josefa / Ur Rahman, Faiz / UR, FAIZ
    Paraules clau: Water Taxonomy Spp. Sp-nov. Prevalence Genetic diversity Cryaerophilus Campylobacter Butzleri Bacteria
    Resum: Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter-like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter-like spp. from marine and shellfish matrices. For this purpose, 14 different Arcobacter-like spp. mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD420nm) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences (p?<?0.05) among broths were calculated by ANOVA. The results indicated that shellfish-associated Arcobacter-like species growth was significantly higher in Arcobacter broth +?50% ASW and the same media supplemented with lyophilized oysters. This is the first study to have used fresh or lyophilized oyster flesh in the enrichment broth for isolation of shellfish-associated Arcobacter-like spp.
    Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 20452322
    Adreça de correu electrònic de l'autor: mariajose.figueras@urv.cat faiz.urrahman@estudiants.urv.cat
    Identificador de l'autor: 0000-0002-2268-8980
    Data d'alta del registre: 2023-02-23
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.nature.com/articles/s41598-020-71442-8
    Referència a l'article segons font original: Scientific Reports. 10 (1): 14547-
    Referència de l'ítem segons les normes APA: Faiz Ur Rahman, Karl B. Andree, Nuria Salas-Massó, Margarita Fernandez-Tejedor, Anna Sanjuan, Maria J. Figueras & M. Dolors Furones (2020). Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment. Scientific Reports, 10(1), 14547-. DOI: 10.1038/s41598-020-71442-8
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.1038/s41598-020-71442-8
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2020
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Multidisciplinary,Multidisciplinary Sciences
    Water
    Taxonomy
    Spp.
    Sp-nov.
    Prevalence
    Genetic diversity
    Cryaerophilus
    Campylobacter
    Butzleri
    Bacteria
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Psicología
    Odontología
    Nutrição
    Multidisciplinary sciences
    Multidisciplinary
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Letras / linguística
    Interdisciplinar
    Geografía
    Geociências
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Educação física
    Educação
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biodiversidade
    Astronomia / física
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