Author, as appears in the article.: Faiz Ur Rahman, Karl B. Andree, Nuria Salas-Massó, Margarita Fernandez-Tejedor, Anna Sanjuan, Maria J. Figueras & M. Dolors Furones
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Figueras Salvat, María Josefa / Ur Rahman, Faiz / UR, FAIZ
Keywords: Water Taxonomy Spp. Sp-nov. Prevalence Genetic diversity Cryaerophilus Campylobacter Butzleri Bacteria
Abstract: Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter-like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter-like spp. from marine and shellfish matrices. For this purpose, 14 different Arcobacter-like spp. mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD420nm) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences (p?<?0.05) among broths were calculated by ANOVA. The results indicated that shellfish-associated Arcobacter-like species growth was significantly higher in Arcobacter broth +?50% ASW and the same media supplemented with lyophilized oysters. This is the first study to have used fresh or lyophilized oyster flesh in the enrichment broth for isolation of shellfish-associated Arcobacter-like spp.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 20452322
Author's mail: mariajose.figueras@urv.cat faiz.urrahman@estudiants.urv.cat
Author identifier: 0000-0002-2268-8980
Record's date: 2023-02-23
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.nature.com/articles/s41598-020-71442-8
Papper original source: Scientific Reports. 10 (1): 14547-
APA: Faiz Ur Rahman, Karl B. Andree, Nuria Salas-Massó, Margarita Fernandez-Tejedor, Anna Sanjuan, Maria J. Figueras & M. Dolors Furones (2020). Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment. Scientific Reports, 10(1), 14547-. DOI: 10.1038/s41598-020-71442-8
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1038/s41598-020-71442-8
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV