Articles producció científica> Ciències Mèdiques Bàsiques

Performance of the digene LQ, RH and PS HPVs genotyping systems on clinical samples and comparison with HC2 and PCR-based Linear Array

  • Dades identificatives

    Identificador: imarina:835446
    Autors:
    Godinez, Jose MTous, SaraBaixeras, NuriaMoreno-Crespi, JudithAlejo, MariaLejeune, MaryleneBravo, Ignacio GBosch, F Xavierde Sanjose, Silvia
    Resum:
    Certain Human Papillomaviruses (HPVs) are the infectious agents involved in cervical cancer development. Detection of HPVs DNA is part of the cervical cancer screening protocols and HPVs genotyping has been proposed for its inclusion in these preventive programs. The aim of this study was to evaluate three novel genotyping tests, namely Qiagen LQ, RH and PS, in clinical samples with and without abnormalities. For this, 305 cervical samples were processed and the results of the evaluated techniques were compared with those obtained in the HPVs diagnostic process in our lab, by using HC2 and Linear Array (LA) technologies.The concordances and kappa statistics (k) for each technique compared with HC2 were 98.69% (k = 0.94) for LQ, 98.03% (k = 0.91) for RH and 91.80% (k = 0.82) for PS. There was a very good agreement in HPVs type-specific concordance for the most prevalent types HPV16 (kappa range = 0.83-0.90), HPV18 (k.r.= 0.74-0.80) and HPV45 (k.r.= 0.82-0.90).The three tests showed an overall good concordance for HPVs detection when compared with HR-HC2 system. LQ and RH rendered lower detection rate for multiple infections than LA genotyping. However, our understanding of the clinical significance of multiple HPVs infections is still incomplete and therefore the relevance of the lower ability to detect multiple infections needs to be evaluated.
  • Altres:

    Autor segons l'article: Godinez, Jose M; Tous, Sara; Baixeras, Nuria; Moreno-Crespi, Judith; Alejo, Maria; Lejeune, Marylene; Bravo, Ignacio G; Bosch, F Xavier; de Sanjose, Silvia
    Departament: Ciències Mèdiques Bàsiques
    Autor/s de la URV: Lejeune, Marylène Marie
    Paraules clau: Papillomaviruses Hpv Genotyping Cervical cancer screening
    Resum: Certain Human Papillomaviruses (HPVs) are the infectious agents involved in cervical cancer development. Detection of HPVs DNA is part of the cervical cancer screening protocols and HPVs genotyping has been proposed for its inclusion in these preventive programs. The aim of this study was to evaluate three novel genotyping tests, namely Qiagen LQ, RH and PS, in clinical samples with and without abnormalities. For this, 305 cervical samples were processed and the results of the evaluated techniques were compared with those obtained in the HPVs diagnostic process in our lab, by using HC2 and Linear Array (LA) technologies.The concordances and kappa statistics (k) for each technique compared with HC2 were 98.69% (k = 0.94) for LQ, 98.03% (k = 0.91) for RH and 91.80% (k = 0.82) for PS. There was a very good agreement in HPVs type-specific concordance for the most prevalent types HPV16 (kappa range = 0.83-0.90), HPV18 (k.r.= 0.74-0.80) and HPV45 (k.r.= 0.82-0.90).The three tests showed an overall good concordance for HPVs detection when compared with HR-HC2 system. LQ and RH rendered lower detection rate for multiple infections than LA genotyping. However, our understanding of the clinical significance of multiple HPVs infections is still incomplete and therefore the relevance of the lower ability to detect multiple infections needs to be evaluated.
    Àrees temàtiques: Saúde coletiva Química Oncology Medicina iii Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology Farmacia Epidemiology Enfermagem Ciências biológicas iii Ciências biológicas i Ciências ambientais Cancer research Biotecnología Biodiversidade
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 17509378
    Adreça de correu electrònic de l'autor: marylenemarie.lejeune@urv.cat
    Identificador de l'autor: 0000-0001-8441-9404
    Data d'alta del registre: 2024-10-12
    Volum de revista: 6
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://infectagentscancer.biomedcentral.com/articles/10.1186/1750-9378-6-23
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Infectious Agents And Cancer. 6 (1): 23-
    Referència de l'ítem segons les normes APA: Godinez, Jose M; Tous, Sara; Baixeras, Nuria; Moreno-Crespi, Judith; Alejo, Maria; Lejeune, Marylene; Bravo, Ignacio G; Bosch, F Xavier; de Sanjose, S (2011). Performance of the digene LQ, RH and PS HPVs genotyping systems on clinical samples and comparison with HC2 and PCR-based Linear Array. Infectious Agents And Cancer, 6(1), 23-. DOI: 10.1186/1750-9378-6-23
    DOI de l'article: 10.1186/1750-9378-6-23
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2011
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Cancer Research,Epidemiology,Immunology,Infectious Diseases,Oncology
    Papillomaviruses
    Hpv
    Genotyping
    Cervical cancer screening
    Saúde coletiva
    Química
    Oncology
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Infectious diseases
    Immunology
    Farmacia
    Epidemiology
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas i
    Ciências ambientais
    Cancer research
    Biotecnología
    Biodiversidade
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