Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Positional Enrichment by Proton Analysis (PEPA): A One?Dimensional 1H?NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics

  • Dades identificatives

    Identificador: imarina:9011166
    Autors:
    Maria VinaixaMiguel A RodríguezSuvi AivioJordi CapelladesJosep GómezNicolau CanyellasTravis H StrackerOscar Yanes
    Resum:
    A novel metabolomics approach for NMR?based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C?satellite peaks using 1D?1H?NMR spectra. In comparison with 13C?NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high?throughput of 1H?NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D?NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
  • Altres:

    Autor segons l'article: Maria Vinaixa; Miguel A Rodríguez; Suvi Aivio; Jordi Capellades; Josep Gómez; Nicolau Canyellas; Travis H Stracker; Oscar Yanes
    Departament: Bioquímica i Biotecnologia Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/s de la URV: Cañellas Alberich, Nicolau / Gómez Alvarez, Josep / Rodríguez Gómez, Miquel Àngel / Vinaixa Crevillent, Maria / Yanes Torrado, Óscar
    Resum: A novel metabolomics approach for NMR?based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C?satellite peaks using 1D?1H?NMR spectra. In comparison with 13C?NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high?throughput of 1H?NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D?NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
    Àrees temàtiques: Química Ciências biológicas iii Astronomia / física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: maria.vinaixa@urv.cat miquelangel.rodriguez@alumni.urv.cat nicolau.canyellas@urv.cat oscar.yanes@urv.cat josep.gomez@urv.cat
    Identificador de l'autor: 0000-0001-9804-0171 0000-0003-4856-8132 0000-0003-3695-7157 0000-0002-0573-7621
    Data d'alta del registre: 2023-09-05
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201611347
    Referència a l'article segons font original: Angewandte Chemie. 129 (13): 3585-3589
    Referència de l'ítem segons les normes APA: Maria Vinaixa; Miguel A Rodríguez; Suvi Aivio; Jordi Capellades; Josep Gómez; Nicolau Canyellas; Travis H Stracker; Oscar Yanes (2017). Positional Enrichment by Proton Analysis (PEPA): A One?Dimensional 1H?NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics. Angewandte Chemie, 129(13), 3585-3589. DOI: 10.1002/ange.201611347
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.1002/ange.201611347
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2017
    Tipus de publicació: Journal Publications
  • Paraules clau:

    metabolism; metabolomics; NMR spectroscopy; stable isotopes
    Química
    Ciências biológicas iii
    Astronomia / física
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