Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Positional Enrichment by Proton Analysis (PEPA): A One?Dimensional 1H?NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics

  • Datos identificativos

    Identificador: imarina:9011166
    Autores:
    Maria VinaixaMiguel A RodríguezSuvi AivioJordi CapelladesJosep GómezNicolau CanyellasTravis H StrackerOscar Yanes
    Resumen:
    A novel metabolomics approach for NMR?based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C?satellite peaks using 1D?1H?NMR spectra. In comparison with 13C?NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high?throughput of 1H?NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D?NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
  • Otros:

    Autor según el artículo: Maria Vinaixa; Miguel A Rodríguez; Suvi Aivio; Jordi Capellades; Josep Gómez; Nicolau Canyellas; Travis H Stracker; Oscar Yanes
    Departamento: Bioquímica i Biotecnologia Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Cañellas Alberich, Nicolau / Gómez Alvarez, Josep / Rodríguez Gómez, Miquel Àngel / Vinaixa Crevillent, Maria / Yanes Torrado, Óscar
    Resumen: A novel metabolomics approach for NMR?based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C?satellite peaks using 1D?1H?NMR spectra. In comparison with 13C?NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high?throughput of 1H?NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D?NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
    Áreas temáticas: Química Ciências biológicas iii Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: maria.vinaixa@urv.cat miquelangel.rodriguez@alumni.urv.cat nicolau.canyellas@urv.cat oscar.yanes@urv.cat josep.gomez@urv.cat
    Identificador del autor: 0000-0001-9804-0171 0000-0003-4856-8132 0000-0003-3695-7157 0000-0002-0573-7621
    Fecha de alta del registro: 2023-09-05
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201611347
    Referencia al articulo segun fuente origial: Angewandte Chemie. 129 (13): 3585-3589
    Referencia de l'ítem segons les normes APA: Maria Vinaixa; Miguel A Rodríguez; Suvi Aivio; Jordi Capellades; Josep Gómez; Nicolau Canyellas; Travis H Stracker; Oscar Yanes (2017). Positional Enrichment by Proton Analysis (PEPA): A One?Dimensional 1H?NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics. Angewandte Chemie, 129(13), 3585-3589. DOI: 10.1002/ange.201611347
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.1002/ange.201611347
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2017
    Tipo de publicación: Journal Publications
  • Palabras clave:

    metabolism; metabolomics; NMR spectroscopy; stable isotopes
    Química
    Ciências biológicas iii
    Astronomia / física
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