Articles producció científica> Psicologia

Paraoxonase-1 and-3 Protein Expression in the Brain of the Tg2576 Mouse Model of Alzheimer's Disease

  • Dades identificatives

    Identificador: imarina:9187123
    Autors:
    Salazar, Jose GregorioMarsillach, JuditReverte, IngridMackness, BhartiMackness, MichaelJoven, JorgeCamps, JordiColomina, Maria Teresa
    Resum:
    Background: Brain oxidative lipid damage and inflammation are common in neurodegenerative diseases such as Alzheimer's disease (AD). Paraoxonase-1 and -3 (PON1 and PON3) protein expression was demonstrated in tissue with no PON1 or PON3 gene expression. In the present study, we examine differences in PON1 and PON3 protein expression in the brain of a mouse model of AD. Methods: we used peroxidase- and fluorescence-based immunohistochemistry in five brain regions (olfactory bulb, forebrain, posterior midbrain, hindbrain and cerebellum) of transgenic (Tg2576) mice with the Swedish mutation (KM670/671NL) responsible for a familial form of AD and corresponding wild-type mice. Results: We found intense PON1 and PON3-positive staining in star-shaped cells surrounding A beta plaques in all the studied Tg2576 mouse-brain regions. Although we could not colocalize PON1 and PON3 with astrocytes (star-shaped cells in the brain), we found some PON3 colocalization with microglia. Conclusions: These results suggest that (1) PON1 and PON3 cross the blood-brain barrier in discoidal high-density lipoproteins (HDLs) and are transferred to specific brain-cell types; and (2) PON1 and PON3 play an important role in preventing oxidative stress and lipid peroxidation in particular brain-cell types (likely to be glial cells) in AD pathology and potentially in other neurodegenerative diseases as well.
  • Altres:

    Autor segons l'article: Salazar, Jose Gregorio; Marsillach, Judit; Reverte, Ingrid; Mackness, Bharti; Mackness, Michael; Joven, Jorge; Camps, Jordi; Colomina, Maria Teresa;
    Departament: Psicologia
    Autor/s de la URV: Colomina Fosch, Maria Teresa / Joven Maried, Jorge / MARSILLACH LÓPEZ, JUDIT / REVERTÉ SOLER, INGRID
    Paraules clau: Tg2576 mice S disease Paraoxonases Oxidative stress Neurons Microglia Hippocampus Brain Astrocytes Amyloid-β Amyloid-? Alzheimer’ Alzheimer’s disease
    Resum: Background: Brain oxidative lipid damage and inflammation are common in neurodegenerative diseases such as Alzheimer's disease (AD). Paraoxonase-1 and -3 (PON1 and PON3) protein expression was demonstrated in tissue with no PON1 or PON3 gene expression. In the present study, we examine differences in PON1 and PON3 protein expression in the brain of a mouse model of AD. Methods: we used peroxidase- and fluorescence-based immunohistochemistry in five brain regions (olfactory bulb, forebrain, posterior midbrain, hindbrain and cerebellum) of transgenic (Tg2576) mice with the Swedish mutation (KM670/671NL) responsible for a familial form of AD and corresponding wild-type mice. Results: We found intense PON1 and PON3-positive staining in star-shaped cells surrounding A beta plaques in all the studied Tg2576 mouse-brain regions. Although we could not colocalize PON1 and PON3 with astrocytes (star-shaped cells in the brain), we found some PON3 colocalization with microglia. Conclusions: These results suggest that (1) PON1 and PON3 cross the blood-brain barrier in discoidal high-density lipoproteins (HDLs) and are transferred to specific brain-cell types; and (2) PON1 and PON3 play an important role in preventing oxidative stress and lipid peroxidation in particular brain-cell types (likely to be glial cells) in AD pathology and potentially in other neurodegenerative diseases as well.
    Àrees temàtiques: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: jorge.joven@urv.cat mariateresa.colomina@urv.cat
    Identificador de l'autor: 0000-0003-2749-4541 0000-0002-5619-4874
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/2076-3921/10/3/339
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Antioxidants. 10 (3): 339-12
    Referència de l'ítem segons les normes APA: Salazar, Jose Gregorio; Marsillach, Judit; Reverte, Ingrid; Mackness, Bharti; Mackness, Michael; Joven, Jorge; Camps, Jordi; Colomina, Maria Teresa; (2021). Paraoxonase-1 and-3 Protein Expression in the Brain of the Tg2576 Mouse Model of Alzheimer's Disease. Antioxidants, 10(3), 339-12. DOI: 10.3390/antiox10030339
    DOI de l'article: 10.3390/antiox10030339
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2021
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemistry,Biochemistry & Molecular Biology,Cell Biology,Chemistry, Medicinal,Clinical Biochemistry,Food Science,Food Science & Technology,Molecular Biology,Physiology
    Tg2576 mice
    S disease
    Paraoxonases
    Oxidative stress
    Neurons
    Microglia
    Hippocampus
    Brain
    Astrocytes
    Amyloid-β Amyloid-?
    Alzheimer’ Alzheimer’s disease
    Química
    Physiology
    Molecular biology
    Medicina ii
    Medicina i
    Interdisciplinar
    Food science & technology
    Food science
    Farmacia
    Engenharias ii
    Clinical biochemistry
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, medicinal
    Cell biology
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Biochemistry
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