Author, as appears in the article.: Salazar, Jose Gregorio; Marsillach, Judit; Reverte, Ingrid; Mackness, Bharti; Mackness, Michael; Joven, Jorge; Camps, Jordi; Colomina, Maria Teresa;

Department: Psicologia

URV's Author/s: Colomina Fosch, Maria Teresa / Joven Maried, Jorge / REVERTÉ SOLER, INGRID

Keywords: Tg2576 mice S disease Paraoxonases Oxidative stress Neurons Microglia Hippocampus Brain Astrocytes Amyloid-β Amyloid-? Alzheimer’ Alzheimer’s disease

Abstract: Background: Brain oxidative lipid damage and inflammation are common in neurodegenerative diseases such as Alzheimer's disease (AD). Paraoxonase-1 and -3 (PON1 and PON3) protein expression was demonstrated in tissue with no PON1 or PON3 gene expression. In the present study, we examine differences in PON1 and PON3 protein expression in the brain of a mouse model of AD. Methods: we used peroxidase- and fluorescence-based immunohistochemistry in five brain regions (olfactory bulb, forebrain, posterior midbrain, hindbrain and cerebellum) of transgenic (Tg2576) mice with the Swedish mutation (KM670/671NL) responsible for a familial form of AD and corresponding wild-type mice. Results: We found intense PON1 and PON3-positive staining in star-shaped cells surrounding A beta plaques in all the studied Tg2576 mouse-brain regions. Although we could not colocalize PON1 and PON3 with astrocytes (star-shaped cells in the brain), we found some PON3 colocalization with microglia. Conclusions: These results suggest that (1) PON1 and PON3 cross the blood-brain barrier in discoidal high-density lipoproteins (HDLs) and are transferred to specific brain-cell types; and (2) PON1 and PON3 play an important role in preventing oxidative stress and lipid peroxidation in particular brain-cell types (likely to be glial cells) in AD pathology and potentially in other neurodegenerative diseases as well.

Thematic Areas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: mariateresa.colomina@urv.cat jorge.joven@urv.cat

Author identifier: 0000-0002-5619-4874 0000-0003-2749-4541

Record's date: 2023-08-05

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2076-3921/10/3/339

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Antioxidants. 10 (3): 1-12

APA: Salazar, Jose Gregorio; Marsillach, Judit; Reverte, Ingrid; Mackness, Bharti; Mackness, Michael; Joven, Jorge; Camps, Jordi; Colomina, Maria Teresa; (2021). Paraoxonase-1 and-3 Protein Expression in the Brain of the Tg2576 Mouse Model of Alzheimer's Disease. Antioxidants, 10(3), 1-12. DOI: 10.3390/antiox10030339

Article's DOI: 10.3390/antiox10030339

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications