Articles producció científica> Bioquímica i Biotecnologia

Transcriptional regulation of ergosterol biosynthesis genes in response to iron deficiency

  • Dades identificatives

    Identificador: imarina:9280730
    Autors:
    Jordá TBarba-Aliaga MRozès NAlepuz PMartínez-Pastor MTPuig S
    Resum:
    Iron participates as an essential cofactor in the biosynthesis of critical cellular components, including DNA, proteins and lipids. The ergosterol biosynthetic pathway, which is an important target of antifungal treatments, depends on iron in four enzymatic steps. Our results in the model yeast Saccharomyces cerevisiae show that the expression of ergosterol biosynthesis (ERG) genes is tightly modulated by iron availability probably through the iron-dependent variation of sterol and heme levels. Whereas the transcription factors Upc2 and Ecm22 are responsible for the activation of ERG genes upon iron deficiency, the heme-dependent factor Hap1 triggers their Tup1-mediated transcriptional repression. The combined regulation by both activating and repressing regulatory factors allows for the fine-tuning of ERG transcript levels along the progress of iron deficiency, avoiding the accumulation of toxic sterol intermediates and enabling efficient adaptation to rapidly changing conditions. The lack of these regulatory factors leads to changes in the yeast sterol profile upon iron-deficient conditions. Both environmental iron availability and specific regulatory factors should be considered in ergosterol antifungal treatments.
  • Altres:

    Autor segons l'article: Jordá T; Barba-Aliaga M; Rozès N; Alepuz P; Martínez-Pastor MT; Puig S
    Departament: Bioquímica i Biotecnologia
    Autor/s de la URV: Rozès, Nicolas Andre Louis
    Paraules clau: Saccharomyces-cerevisiae yeast rox1 pathway heme expression activator hap1 absence
    Resum: Iron participates as an essential cofactor in the biosynthesis of critical cellular components, including DNA, proteins and lipids. The ergosterol biosynthetic pathway, which is an important target of antifungal treatments, depends on iron in four enzymatic steps. Our results in the model yeast Saccharomyces cerevisiae show that the expression of ergosterol biosynthesis (ERG) genes is tightly modulated by iron availability probably through the iron-dependent variation of sterol and heme levels. Whereas the transcription factors Upc2 and Ecm22 are responsible for the activation of ERG genes upon iron deficiency, the heme-dependent factor Hap1 triggers their Tup1-mediated transcriptional repression. The combined regulation by both activating and repressing regulatory factors allows for the fine-tuning of ERG transcript levels along the progress of iron deficiency, avoiding the accumulation of toxic sterol intermediates and enabling efficient adaptation to rapidly changing conditions. The lack of these regulatory factors leads to changes in the yeast sterol profile upon iron-deficient conditions. Both environmental iron availability and specific regulatory factors should be considered in ergosterol antifungal treatments.
    Àrees temàtiques: Química Microbiology Medicina ii Medicina i Interdisciplinar Geografía Geociências General medicine Farmacia Engenharias iii Engenharias i Ecology, evolution, behavior and systematics Ecology Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: nicolasrozes@urv.cat
    Identificador de l'autor: 0000-0001-9718-3429
    Data d'alta del registre: 2024-09-07
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://ami-journals.onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.16157
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Environmental Microbiology. 24 (11): 5248-5260
    Referència de l'ítem segons les normes APA: Jordá T; Barba-Aliaga M; Rozès N; Alepuz P; Martínez-Pastor MT; Puig S (2022). Transcriptional regulation of ergosterol biosynthesis genes in response to iron deficiency. Environmental Microbiology, 24(11), 5248-5260. DOI: 10.1111/1462-2920.16157
    DOI de l'article: 10.1111/1462-2920.16157
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2022
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Ecology,Ecology, Evolution, Behavior and Systematics,Microbiology
    Saccharomyces-cerevisiae
    yeast
    rox1
    pathway
    heme
    expression
    activator hap1
    absence
    Química
    Microbiology
    Medicina ii
    Medicina i
    Interdisciplinar
    Geografía
    Geociências
    General medicine
    Farmacia
    Engenharias iii
    Engenharias i
    Ecology, evolution, behavior and systematics
    Ecology
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biodiversidade
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