Autor según el artículo: Jordá T; Barba-Aliaga M; Rozès N; Alepuz P; Martínez-Pastor MT; Puig S
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Rozès, Nicolas Andre Louis
Palabras clave: Saccharomyces-cerevisiae yeast rox1 pathway heme expression activator hap1 absence
Resumen: Iron participates as an essential cofactor in the biosynthesis of critical cellular components, including DNA, proteins and lipids. The ergosterol biosynthetic pathway, which is an important target of antifungal treatments, depends on iron in four enzymatic steps. Our results in the model yeast Saccharomyces cerevisiae show that the expression of ergosterol biosynthesis (ERG) genes is tightly modulated by iron availability probably through the iron-dependent variation of sterol and heme levels. Whereas the transcription factors Upc2 and Ecm22 are responsible for the activation of ERG genes upon iron deficiency, the heme-dependent factor Hap1 triggers their Tup1-mediated transcriptional repression. The combined regulation by both activating and repressing regulatory factors allows for the fine-tuning of ERG transcript levels along the progress of iron deficiency, avoiding the accumulation of toxic sterol intermediates and enabling efficient adaptation to rapidly changing conditions. The lack of these regulatory factors leads to changes in the yeast sterol profile upon iron-deficient conditions. Both environmental iron availability and specific regulatory factors should be considered in ergosterol antifungal treatments.
Áreas temáticas: Química Microbiology Medicina ii Medicina i Interdisciplinar Geografía Geociências General medicine Farmacia Engenharias iii Engenharias i Ecology, evolution, behavior and systematics Ecology Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: nicolasrozes@urv.cat
Identificador del autor: 0000-0001-9718-3429
Fecha de alta del registro: 2023-02-23
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://ami-journals.onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.16157
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Environmental Microbiology. 24 (11): 5248-5260
Referencia de l'ítem segons les normes APA: Jordá T; Barba-Aliaga M; Rozès N; Alepuz P; Martínez-Pastor MT; Puig S (2022). Transcriptional regulation of ergosterol biosynthesis genes in response to iron deficiency. Environmental Microbiology, 24(11), 5248-5260. DOI: 10.1111/1462-2920.16157
DOI del artículo: 10.1111/1462-2920.16157
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications
Repositori URV