Repositori URV

Identificador: imarina:9283412

Handle: https://hdl.handle.net/20.500.11797/imarina9283412

Autors:
Valera-Garrido FMargalef RBosque MMinaya-Muñoz FSantafé MM

Resum:
Muscle regeneration after a traumatic injury can take an excessively long period of time. The purpose of this study is to assess whether the action of percutaneous needle electrolysis (PNE) accelerates muscle regeneration in cases of partial muscle injuries. The gastrocnemius muscle from adult Swiss male mice was inoculated with bupivacaine. The PNE protocol was applied 48 h after treatment with bupivacaine. Immunofluorescence techniques were performed 72 h after treatment with bupivacaine to evaluate the synaptic contacts. The end plate noise was recorded by electromyography after treatment with bupivacaine. Bupivacaine induced a local injury in muscles, axons were retracted, and the endplate noise decreased at 72 h, while the endplate noise increased in the injured limb where PNE had been applied. Seven days later, the functional values were the same as the controls and they were maintained for 10 days. The endplate noise was significantly greater on the limb treated with the electric current when compared to the limb receiving only bupivacaine, indicating that the use of galvanic current facilitated muscle regeneration at least from a functional point of view. The application of PNE during muscle regeneration in an animal model reduces the recovery time of the damaged muscle tissue.