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Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium

  • Dades identificatives

    Identificador: imarina:9292253
    Autors:
    Gunasekaran, VijayalakshmiCanela, NuriaConstanti, Magda
    Resum:
    A bacterial consortium capable of degrading ethyl tert-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. Arthrobacter sp., Herbaspirillum sp., Pseudacidovorax sp., Pseudomonas sp., and Xanthomonas sp. were identified as the initial populations with the 16S rDNA analysis. The consortium aerobically degraded 49% of 50 mg/L of ETBE, in 6 days. The ETBE degrading efficiency of the consortium increased to 98% even with the higher concentrations of ETBE (1000 mg/L) in the subsequent subcultures, which accumulated tert-butyl alcohol (TBA). Xanthomonas sp. and Pseudomonas sp. were identified as the predominant ETBE degrading populations in the final subculture. The metaproteome of the ETBE-grown bacterial consortium was compared with the glucose-grown bacterial consortium, using 2D-DIGE. Proteins related to the ETBE metabolism, stress response, carbon metabolism and chaperones were found to be abundant in the presence of ETBE while proteins related to cell division were less abundant. The metaproteomic study revealed that the ETBE does have an effect on the metabolism of the bacterial consortium. It also enabled us to understand the responses of the complex bacterial consortium to ETBE, thus revealing interesting facts about the ETBE degrading bacterial community.
  • Altres:

    Autor segons l'article: Gunasekaran, Vijayalakshmi; Canela, Nuria; Constanti, Magda;
    Departament: Enginyeria Química
    Autor/s de la URV: Constantí Garriga, Magdalena / GUNASEKARAN, VIJAYALAKSHMI
    Paraules clau: Strain Sp nov. Pathway Mtbe Metaproteome Inhalation exposure Hydrocarbons Herbaspirillum-chlorophenolicum Etbe Degradation Biodegradation Bacterial consortium 2d-dige
    Resum: A bacterial consortium capable of degrading ethyl tert-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. Arthrobacter sp., Herbaspirillum sp., Pseudacidovorax sp., Pseudomonas sp., and Xanthomonas sp. were identified as the initial populations with the 16S rDNA analysis. The consortium aerobically degraded 49% of 50 mg/L of ETBE, in 6 days. The ETBE degrading efficiency of the consortium increased to 98% even with the higher concentrations of ETBE (1000 mg/L) in the subsequent subcultures, which accumulated tert-butyl alcohol (TBA). Xanthomonas sp. and Pseudomonas sp. were identified as the predominant ETBE degrading populations in the final subculture. The metaproteome of the ETBE-grown bacterial consortium was compared with the glucose-grown bacterial consortium, using 2D-DIGE. Proteins related to the ETBE metabolism, stress response, carbon metabolism and chaperones were found to be abundant in the presence of ETBE while proteins related to cell division were less abundant. The metaproteomic study revealed that the ETBE does have an effect on the metabolism of the bacterial consortium. It also enabled us to understand the responses of the complex bacterial consortium to ETBE, thus revealing interesting facts about the ETBE degrading bacterial community.
    Àrees temàtiques: Virology Microbiology (medical) Microbiology
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: magdalena.constanti@urv.cat
    Identificador de l'autor: 0000-0002-3547-4199
    Data d'alta del registre: 2024-09-07
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/2076-2607/10/12/2331
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Microorganisms. 10 (12):
    Referència de l'ítem segons les normes APA: Gunasekaran, Vijayalakshmi; Canela, Nuria; Constanti, Magda; (2022). Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium. Microorganisms, 10(12), -. DOI: 10.3390/microorganisms10122331
    DOI de l'article: 10.3390/microorganisms10122331
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2022
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Microbiology,Microbiology (Medical),Virology
    Strain
    Sp nov.
    Pathway
    Mtbe
    Metaproteome
    Inhalation exposure
    Hydrocarbons
    Herbaspirillum-chlorophenolicum
    Etbe
    Degradation
    Biodegradation
    Bacterial consortium
    2d-dige
    Virology
    Microbiology (medical)
    Microbiology
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