Autor segons l'article: Boronat-Toscano, A; Vañó, I; Monfort-Ferré, D; Menacho, M; Valldosera, G; Caro, A; Espina, B; Mañas, MJ; Marti, M; Espin, E; Saera-Vila, A; Serena, C
Departament: Medicina i Cirurgia
Autor/s de la URV: Boronat Toscano, Albert / Menacho Viladot, Margarita / Monfort Ferre, Diandra / Serena Perelló, Carolina
Paraules clau: Tobacco Macrophages Inflammatory bowel diseases Immune cells Dna methylation Differentially methylated regions Cigarette Chronic inflammatory disease Cell therapy Adipose tissue tobacco macrophages inflammatory bowel diseases immune cells differentially methylated regions cigarette chronic inflammatory disease cell therapy adipose tissue
Resum: Patients with Crohn’s disease (CD) who smoke are known to have a worse prognosis than never-smokers and a higher risk for post-surgical recurrence, whereas patients who quit smoking after surgery have significantly lower post-operative recurrence. The hypothesis was that smoking induces epigenetic changes that impair the capacity of adipose stem cells (ASCs) to suppress the immune system. It was also questioned whether this impairment remains in ex-smokers with CD. ASCs were isolated from non-smokers, smokers and ex-smokers with CD and their interactions with immune cells were studied. The ASCs from both smokers and ex-smokers promoted macrophage polarization to an M1 pro-inflammatory phenotype, were not able to inhibit T- and B-cell proliferation in vitro and enhanced the gene and protein expression of inflammatory markers including interleukin-1b. Genome-wide epigenetic analysis using two different bioinformatic approaches revealed significant changes in the methylation patterns of genes that are critical for wound healing, immune and metabolic response and p53-mediated DNA damage response in ASCs from smokers and ex-smokers with CD. In conclusion, cigarette smoking induces a pro-inflammatory epigenetic signature in ASCs that likely compromises their therapeutic potential.
Àrees temàtiques: Medicine (miscellaneous) Cell biology Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: margarita.menacho@urv.cat carolina.serena@urv.cat albert.boronat@estudiants.urv.cat diandra.monfort@estudiants.urv.cat
Identificador de l'autor: 0000-0003-3832-4249
Data d'alta del registre: 2024-08-03
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.mdpi.com/2073-4409/12/7/1021
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Cells. 12 (7): 1021-
Referència de l'ítem segons les normes APA: Boronat-Toscano, A; Vañó, I; Monfort-Ferré, D; Menacho, M; Valldosera, G; Caro, A; Espina, B; Mañas, MJ; Marti, M; Espin, E; Saera-Vila, A; Serena, C (2023). Smoking Suppresses the Therapeutic Potential of Adipose Stem Cells in Crohn’s Disease Patients through Epigenetic Changes. Cells, 12(7), 1021-. DOI: 10.3390/cells12071021
DOI de l'article: 10.3390/cells12071021
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2023
Tipus de publicació: Journal Publications